Detects human Frizzled-6 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Frizzled-6 is observed, and less than 5% cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, recombinant human (rh) Frizzled-5, rmFrizzled-7, rmFrizzled-8, and rhFrizzled-10 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Frizzled-6 His19-Val153 Accession # O60353
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human Frizzled‑6
2.5 µg/106 cells
HEK293 human embryonic kidney cell line
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Frizzled‑6 in Human Kidney Cancer Tissue. Frizzled‑6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein
coupled receptors that mediate the Wnt beta-Catenin signaling pathway.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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species: Human.We have 3 review tested in 1 application: Immunocytochemistry/Immunofluorescence.