Human Frizzled-6 Antibody

Catalog # Availability Size / Price Qty
AF3149
AF3149-SP
Frizzled‑6 in Human Kidney Cancer Tissue.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (3)

Human Frizzled-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Frizzled-6 in direct ELISAs and Western blots.  In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Frizzled-6 is observed, and less than 5% cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, recombinant human (rh) Frizzled-5, rmFrizzled-7, rmFrizzled-8, and rhFrizzled-10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Frizzled-6
His19-Val153
Accession # O60353
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Frizzled‑6
Flow Cytometry
2.5 µg/106 cells
HEK293 human embryonic kidney cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Frizzled‑6 in Human Kidney Cancer Tissue. View Larger

Frizzled‑6 in Human Kidney Cancer Tissue. Frizzled‑6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-6

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Entrez Gene IDs
8323 (Human); 14368 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 6; frizzled homolog 6 (Drosophila); Frizzled6; Frizzled-6; FZ6; fz-6; FZD6; Hfz6; seven transmembrane helix receptor

Product Datasheets

Citation for Human Frizzled-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
    Authors: Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A
    BMC Cancer, 2013;13(0):557.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Frizzled-6 Antibody

Average Rating: 5 (Based on 3 Reviews)

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Human Frizzled‑6 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: MDA-MBA-231 human breast cancer cell line Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS with 2%BSA
Dilution: 1:250


Human Frizzled‑6 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: SUM-159PT human breast cancer cell line Species: Human

4% PFA fixation, Secondary Ab: Alexa488-donkey-anti-goat 1/500.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS/Tween
Dilution: 1/250


Human Frizzled‑6 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Cells were fixed in 4% PFA. Secondary Ab: Alexa488-donkey-anti-goat 1/500.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS/Tween
Dilution: 1/250