Human Frizzled-6 Antibody Summary
Accession # O60353
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Frizzled‑6 in Human Kidney Cancer Tissue. Frizzled-6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.
Citation for Human Frizzled-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
Authors: Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A
BMC Cancer, 2013;13(0):557.
Sample Types: Whole Cells
Applications: Flow Cytometry
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Cell and Tissue Staining Kits
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Buffer: PBS with 2%BSA
4% PFA fixation, Secondary Ab: Alexa488-donkey-anti-goat 1/500.
Cells were fixed in 4% PFA. Secondary Ab: Alexa488-donkey-anti-goat 1/500.