Human Frizzled-6 Antibody Summary
Accession # O60353
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Frizzled‑6 in Human Kidney Cancer Tissue. Frizzled‑6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.
Citation for Human Frizzled-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
Authors: Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A
BMC Cancer, 2013;13(0):557.
Sample Types: Whole Cells
Applications: Flow Cytometry
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Cell and Tissue Staining Kits
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Buffer: PBS with 2%BSA
4% PFA fixation, Secondary Ab: Alexa488-donkey-anti-goat 1/500.
Cells were fixed in 4% PFA. Secondary Ab: Alexa488-donkey-anti-goat 1/500.