Human FUS Antibody (1120264))

Detection of Human FUS by Western Blot.

Western Blot shows lysates of Daudi human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human FUS Monoclonal Antibody (Catalog # MAB11793) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for FUS at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immersion fixed paraffin-embedded sections of human testis

FUS was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human FUS Monoclonal Antibody (Catalog # MAB11793) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human FUS by Simple Western^TM.

Left: Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.1 mg/ml. A specific band was detected for FUS at approximately 101 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Mouse Anti-Human FUS Monoclonal Antibody (Catalog # MAB11793) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human FUS Monoclonal Antibody (Catalog # MAB11793) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the Daudi human Burkitt's lymphoma cell line.