GAD2/GAD65 in Human Dorsal Root Ganglia. GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Glutamic acid decarboxylases (GAD), which catalyze the formation of
gamma-aminobutyric acid from L-glutamic acid, are detectable in
different isoforms encoded by distinct genes. GAD2, also known as
GAD65, is expressed in the brain and pancreatic islets. GAD2 has been
implicated as an autoantigen in the autoimmune disease Stiff Man
Syndrome and in insulin-dependent diabetes mellitus.
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)
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