Human GAD2/GAD65 Antibody Summary
Accession # Q05329
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
GAD2/GAD65 in Human Dorsal Root Ganglia. GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Glutamic acid decarboxylases (GAD), which catalyze the formation of gamma-aminobutyric acid from L-glutamic acid, are detectable in different isoforms encoded by distinct genes. GAD2, also known as GAD65, is expressed in the brain and pancreatic islets. GAD2 has been implicated as an autoantigen in the autoimmune disease Stiff Man Syndrome and in insulin-dependent diabetes mellitus.
Citations for Human GAD2/GAD65 Antibody
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Citations: Showing 1 - 3
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Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR
Authors: FJ Cortez, D Gebhart, PV Robinson, D Seftel, N Pourmandi, J Owyoung, CR Bertozzi, DM Wilson, DM Maahs, BA Buckingham, JR Mills, MM Roforth, SJ Pittock, A McKeon, K Page, WA Wolf, S Sanda, C Speake, CJ Greenbaum, CT Tsai
PLoS ONE, 2020;15(11):e0242049.
Sample Types: Serum
Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells
Authors: Y Miura, MY Li, F Birey, K Ikeda, O Revah, MV Thete, JY Park, A Puno, SH Lee, MH Porteus, SP Pa?ca
Nature Biotechnology, 2020;38(12):1421-1430.
Sample Types: Whole Cells
Prolonged human neural stem cell maturation supports recovery in injured rodent CNS
Authors: P Lu, S Ceto, Y Wang, L Graham, D Wu, H Kumamaru, E Staufenber, MH Tuszynski
J. Clin. Invest., 2017;0(0):.
Sample Types: Whole Cells
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