Human Gas6 Antibody AF885: R&D Systems

Human Gas6 Antibody

(10 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Gas6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Gas6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Gas6
    Asp118-Ala678
    Accession # NP_000811
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Gas6 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and human intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Gas6 at approximately 64 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Gas6 in Human Stomach Cancer Tissue. Gas6 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using 10 µg/mL Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% amino acid (aa) sequence identity with mouse and rat Gas6 and 43% aa sequence identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF‑induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

  • References:
    1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
    2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
    3. Stitt, T.N. et al. (1995) Cell 80:661.
    4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
    5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
    6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
    7. Sather, S. et al. (2007) Blood 109:1026.
    8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
    9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
    10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
    11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
    12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
    13. Gallicchio, M. et al. (2005) Blood 105:1970.
    14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
    15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
    16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
  • Long Name:
    Growth-arrest-specific Protein 6
  • Entrez Gene IDs:
    2621 (Human); 14456 (Mouse)
  • Alternate Names:
    AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Sympathetic signaling reactivates quiescent�disseminated prostate cancer cells in the bone marrow
    Authors: A Decker, Y Jung, FC Cackowski, K Yumoto, J Wang, RS Taichman
    Mol. Cancer Res., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids?
    Cardiovasc Res, 2013;97(1):125-33.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  3. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012;189(7):3508-20.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  5. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011;107(1):140-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques.
    Authors: Hurtado B, Munoz X, Recarte-Pelz P, Garcia N, Luque A, Krupinski J, Sala N, Garcia de Frutos P
    Thromb. Haemost., 2011;105(5):873-82.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  7. GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche.
    Authors: Shiozawa Y, Pedersen EA, Taichman RS
    Exp. Hematol., 2010;38(2):132-40.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
    Authors: Hung YJ, Lee CH, Chu NF
    Diabetes Care, 2010;33(8):1840-4.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  9. Gas6 evaluation in patients with acute dyspnea due to suspected pulmonary embolism.
    Authors: Sainaghi PP, Alciato F, Carnieletto S, Castello L, Bergamasco L, Sola D, Bongo AS, Inglese E, Polosa R, Avanzi GC
    Respir Med, 2009;103(4):589-94.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  10. Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions.
    Authors: Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B
    Am. J. Pathol., 2009;175(1):283-93.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
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