Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1065032
View all GDF-5/BMP-14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GDF-5
Ala382-Arg501
Accession # P43026

Specificity

Detects human GDF-5 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GDF‑5/BMP‑14 Antibody

Detection of GDF‑5/BMP‑14 in U-118MG (positive) and MCF-7 (negative) cells.

GDF‑5/BMP‑14 was detected in immersion fixed U‑118 MG human glioblastoma/astrocytoma cells (positive) and absent in MCF-7 MG human glioblastoma cells (negative) using Mouse Anti-Human GDF‑5/BMP‑14 Monoclonal Antibody (Catalog # MAB8340) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GDF‑5/BMP‑14 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U-118 MG human glioblastoma/astrocytoma cells (positive) and MCF-7 MG human glioblastoma cells (negative) 

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1) and BMP-14, is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF‑ beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N‑terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a subgroup within the BMP family. Like other TGF‑ beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
  6. Inada, M. et al. (1996) BBRC 222:317.

Long Name

Growth Differentiation Factor 5

Alternate Names

BMP-14, CDMP-1, GDF5

Entrez Gene IDs

8200 (Human); 14563 (Mouse)

Gene Symbol

GDF5

UniProt

P43026

Additional GDF-5/BMP-14 Products

Product Documents for Human GDF‑5/BMP‑14 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GDF‑5/BMP‑14 Antibody

For research use only

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