Human GITR/TNFRSF18 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human GITR/TNFRSF18 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse GITR and recombiant human 4-1BB is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
    Gln26-Glu161 (Thr45Ala)
    Accession # Q9Y5U5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR)
  • Flow Cytometry
    2.5 µg/106 cells
    Human T cells treated with PHA
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1-2 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Biotinylated Human GITR Ligand/TNFSF18 to immobilized Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
GITR/TNFRSF18 in Human Tonsil. GITR/TNFRSF18 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human GITR/TNFRSF18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF689) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trabeculae sinuses. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

  • References:
    1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
    2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
    3. Gurney, A.L. et al. (1999) Current Biology 9:215.
    4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.
  • Long Name:
    Glucocorticoid Induced TNF Receptor Family Related Gene
  • Entrez Gene IDs:
    8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
  • Alternate Names:
    Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. The prevalence of regulatory T cells in lymphoid tissue is correlated with viral load in HIV-infected patients.
    Authors: Andersson J, Boasso A, Nilsson J, Zhang R, Shire NJ, Lindback S, Shearer GM, Chougnet CA
    J. Immunol., 2005;174(6):3143-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 11
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Anti-Goat HRP-AEC Cell & Tissue Staining Kit

CTS009 7
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 6
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Antigen Retrieval Reagent-Basic

CTS013 3
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Mounting Medium

CTS011 1
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Antigen Retrieval Reagent-Acidic

CTS014
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DAB Enhancer

CTS010 1
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NorthernLights Guard Mounting Media

NL996
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 129  
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF109 12  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF017 11  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 3
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 2  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 4  
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 4
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 2  
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Donkey Anti-Goat IgG Antibody

WB AF109 5
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Goat IgG VisUCyte HRP Polymer Antibody

IHC VC004  
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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