Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 778633
View all Hemoglobin zeta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Hemoglobin zeta
Met1-Arg142
Accession # P02008

Specificity

Detects human Hemoglobin zeta in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Hemoglobin zeta Antibody

Detection of Human Hemoglobin ? antibody by Western Blot.

Detection of Human Hemoglobin zeta by Western Blot.

Western blot shows lysates of TF-1 human erythroleukemic cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Hemoglobin zeta Monoclonal Antibody (Catalog # MAB7708) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hemoglobin zeta at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Hemoglobin ? antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

Hemoglobin zeta in K562 Human Cell Line.

Hemoglobin zeta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human Hemoglobin zeta Monoclonal Antibody (Catalog # MAB7708) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Hemoglobin zeta Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Western Blot

0.2 µg/mL
Sample: TF‑1 human erythroleukemic cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hemoglobin zeta

Hemoglobin is a tetrameric heme-containing protein that is responsible for the transport of oxygen by red blood cells in the circulation. For most of fetal development and adulthood, hemoglobin consists of two alpha chains and two beta chains. Hemoglobin zeta (HBZ) is an approximately 15 kDa alpha chain-like protein that is produced during the first few weeks of embryogenesis until the onset of alpha chain expression. Its expression is prolonged in a0-thalassemia which is characterized by deficient alpha chain production. The Gower-1, Portland-1, and Portland-2 forms of hemoglobin consist of two zeta chains in association with either two epsilon, gamma, or beta chains, respectively. Human Hemoglobin zeta shares 79% amino acid sequence identity with mouse and rat Hemoglobin zeta.

Alternate Names

HBAZ, HBZ, HBZ2, zeta-globin

Entrez Gene IDs

3050 (Human); 15126 (Mouse)

Gene Symbol

HBZ

UniProt

P02008 (Human)

Additional Hemoglobin zeta Products

Product Documents for Human Hemoglobin zeta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Hemoglobin zeta Antibody

For research use only

Related Research Areas

Red Blood Cells (RBCs)

Citations for Human Hemoglobin zeta Antibody

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Protocols

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