Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 713802
View all Histone Deacetylase 4/HDAC4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Histone Deacetylase 4/HDAC4
Met1-Glu68
Accession # P56524

Specificity

Detects human Histone Deacetylase 4/HDAC4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Histone Deacetylase 4/HDAC4 Antibody

Histone Deacetylase 4/HDAC4 antibody in Human Testis by Immunohistochemistry (IHC-P).

Histone Deacetylase 4/HDAC4 in Human Testis.

Histone Deacetylase 4/HDAC4 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Histone Deacetylase 4/HDAC4 Monoclonal Antibody (Catalog # MAB6205) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Histone Deacetylase 4/HDAC4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Reviewed Applications

Read 1 review rated 5 using MAB6205 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone Deacetylase 4/HDAC4

Histone Deacetylase 4 (HDAC4; also HD4) is a founding member of the class IIa subfamily, histone deacetylase family of transcriptional regulators. Although its predicted MW is 119 kDa, it runs anomalously at 140‑150 kDa in SDS‑PAGE which may be due to extensive phosphorylation plus SUMOylation. It has an N-terminal region that interacts with transcription factors and corepressors, and a C-terminal domain that shows deacetylase activity, thus repressing gene transcription. HDAC4 is found in osteoblasts, cardiac and skeletal muscle cells, and neurons. Human HDAC4 is 1084 amino acids (aa) in length. It contains one coiled-coil region (aa 67‑177), a SUMOylation site at Lys559, a histone deacetylation domain (aa 665‑993) and one NES (aa 1051‑1084). Caspase cleavage after Asp289 generates bioactive 97 and 34 kDa fragments. There is one potential splice variant that shows an alternative start site at Met118 coupled to a five aa insertion after Thr431. Over aa 1‑68, human HDAC4 shares 96% aa identity with mouse HDAC4.

Long Name

Histone Deacetylase 4

Alternate Names

HDAC4

Entrez Gene IDs

9759 (Human); 208727 (Mouse); 363287 (Rat)

Gene Symbol

HDAC4

UniProt

P56524 (Human)

Additional Histone Deacetylase 4/HDAC4 Products

Product Documents for Human Histone Deacetylase 4/HDAC4 Antibody

Certificate of Analysis

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Product Specific Notices for Human Histone Deacetylase 4/HDAC4 Antibody

For research use only

Related Research Areas

Adult Neurogenesis
Epigenetics

Customer Reviews for Human Histone Deacetylase 4/HDAC4 Antibody (1)

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  • Human Histone Deacetylase 4/HDAC4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 02/23/2022
    Human Histone Deacetylase 4/HDAC4 Antibody MAB6205

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