Human HLA-DRB1 Antibody

Catalog #: MAB11640 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11640-100
MAB11640-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HLA-DRB1 Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide specific for human HLA-DRB1 around amino acid 45 in Direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1091354

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # P01911

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of HLA DRB1 in Human Tonsil. HLA DRB1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA DRB1 Monoclonal Antibody (Catalog # MAB11640) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HLA-DRB1

HLA-DRB1 is an HLA class II histocompatibility antigen. It is part of a heterodimer consisting of an alpha (DRA) and a beta chain (DRB) which are both anchored in the membrane. Class II molecules are constitutively expressed in professional APC's. HLA-II is an important immune system protein that plays a role in tumor antigen presentation, immune response, and immune surveillance. HLA-DRB1 presents antigenic peptides to HLA-DRB1-restricted CD4+ T-cells. Aberrant expression of HLA-II is closely related to the pathogenesis of tumors.

References

"Entrez Gene: HLA-DRB1 major histocompatibility complex, Class II, DR beta I"
Hu JM, Li L, Chen YZ, Liu C, Cui X, Yin L, Yang L, Zou H, Pang L, Zhao J, Qi Y, Cao Y, Jiang J, Liang W, Li F. HLA-DRB1 and HLA-DQB1 methylation changes promote the occurrence and progression of Kazakh ESCC. Epigenetics. 2014 Oct;9(10):1366-73. doi: 10.4161/15592294.2014.969625. PMID: 25437052; PMCID: PMC4623353.
Zhao X, Li J, Zhu Q, Liang G, Xia W, He X, Zhu C, Qi H, Deng B, Chen X, Zhang J. HLA-A and HLA-DRB1 may play a unique role in ovarian teratoma-associated anti-N-methyl-D-aspartate receptor encephalitis. Reprod Biol Endocrinol. 2020 Nov 7;18(1):107. doi: 10.1186/s12958-020-00661-5. PMID: 33160385; PMCID: PMC7648266.

Long Name

Major histocompatibility complex, class II, DR beta 1

Entrez Gene IDs

3123 (Human)

Alternate Names

Clone P2-beta-3; DR1; DR-1; DR12; DR-12; DR13; DR-13; DR14; DR-14; DR16; DR-16; DR4; DR-4; DR5; DR-5; DR7; DR-7; DR8; DR-8; DR9; DR-9; DRB1; DRw10; DRw11; DRw8; DW2.2/DR2.2; FLJ75017; FLJ76359; HLA class II antigen beta chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA-DR1B; HLA-DRB; HLA-DRB1; HLA-DRB1*; HLA-DRB2; HLA-DR-beta 1; human leucocyte antigen DRB1; leucocyte antigen DR beta 1 chain; leucocyte antigen DRB1; lymphocyte antigen DRB1; major histocompatibility complex, class II, DR beta 1; MHC class II antigen DRB1*1; MHC class II antigen DRB1*10; MHC class II antigen DRB1*11; MHC class II antigen DRB1*12; MHC class II antigen DRB1*13; MHC class II antigen DRB1*14; MHC class II antigen DRB1*15; MHC class II antigen DRB1*16; MHC class II antigen DRB1*3; MHC class II antigen DRB1*4; MHC class II antigen DRB1*7; MHC class II antigen DRB1*8; MHC class II antigen DRB1*9; MHC class II antigen HLA-DR13; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; SS1