Human HSP10/EPF Antibody

Catalog # Availability Size / Price Qty
MAB3298
MAB3298-SP
Detection of Human HSP10/EPF by Western Blot.
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Human HSP10/EPF Antibody Summary

Species Reactivity
Human
Specificity
Detects human HSP10/EPF in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 330629
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HSP10/EPF
Ala2-Asp102
Accession # P61604
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human HSP10/EPF antibody by Western Blot. View Larger

Detection of Human HSP10/EPF by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HSP10/EPF Monoclonal Antibody (Catalog # MAB3298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP10/EPF at approximately 8-9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry HSP10/EPF antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

HSP10/EPF in HeLa Human Cell Line. HSP10/EPF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human HSP10/EPF Monoclonal Antibody (Catalog # MAB3298) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP10/EPF

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP10, also known as HSPE1, chaperonin 10 and Early Pregnancy Factor (EPF), is a 10.8 kDa protein with an apparent molecular weight of 8‑9 kDa that exists intracellularly as a 7 subunit multimer and assists HSP60 in folding non-native proteins.

Long Name
Heat Shock 10 kDa Protein 1
Entrez Gene IDs
3336 (Human); 69253 (Mouse)
Alternate Names
10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); HSP10; HSPE1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human HSP10/EPF Antibody

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Human HSP10/EPF Antibody
By Anonymous on 04/11/2022
Application: WB Sample Tested: HeLa human cervical epithelial carcinoma cell line Species: Human