Human HSP47 Antibody (950811)

R&D Systems | Catalog # MAB91661

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 950811
View all HSP47 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human HSP47
Ala19-Asp412
Accession # P50454

Specificity

Detects human HSP47 in direct ELISAs and Western blots.  In Western blots, no cross-reactivity with mouse HSP47 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human HSP47 Antibody (950811)

Detection of Human HSP47 antibody by Western Blot.

Detection of Human HSP47 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP47 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HSP47 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

HSP47 in BG01V Human Embryonic Stem Cells.

HSP47 was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
HSP47 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

HSP47 in Human Breast Cancer Tissue.

HSP47 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human HSP47 Antibody (950811)

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR‑3 human ovarian carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB91661 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP47

Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.  In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X.  Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.  Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.

References

  1. Christiansen HE, et al, (2010) Am. J. Hum. Genet. 86:3892.
  2. Tasab M, et al, (2000) EMBO J. 19:22043.
  3. Kwon YJ, et al, (2009) Oncol Res. 18:1414.
  4. Zhu J, et al, (2015) Cancer Res. 75:15805.
  5. Nese N, et al, (2010) Anal Quant Cytol Histol. 32:90

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Entrez Gene IDs

871 (Human); 12406 (Mouse); 29345 (Rat)

Gene Symbol

SERPINH1

UniProt

P50454

Additional HSP47 Products

Product Documents for Human HSP47 Antibody (950811)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HSP47 Antibody (950811)

For research use only

Citations for Human HSP47 Antibody (950811)

Customer Reviews for Human HSP47 Antibody (950811) (1)

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  • Human HSP47 Antibody
    Name: Maximilian Haist
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 04/03/2023
    Human Tonsil section stained for HSP47
    Human HSP47 Antibody (950811) MAB91661
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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