Human HTRA2/Omi Antibody Summary
Accession # O43464
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human HTRA2/Omi by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA2/Omi Monoclonal Antibody (Catalog # MAB1458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA2/Omi at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
HTRA2/Omi in K562 Human Cell Line. HTRA2/Omi was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human HTRA2/Omi Monoclonal Antibody (Catalog # MAB1458) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HTRA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HTRA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HTRA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (R&D Systems, Catalog # 786-XB) inhibition of Caspase-7 (R&D Systems, Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HTRA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site.
- Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
- van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
- Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
- Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
- Martins, L. et al. (2002) J. Biol. Chem. 277:439.
- Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
- Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.
Citations for Human HTRA2/Omi Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways.
Authors: Haudek SB, Taffet GE, Schneider MD, Mann DL
J. Clin. Invest., 2007-09-01;117(9):2692-701.
Sample Types: Cell Culture Supernates
Applications: Western Blot
Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins.
Authors: Setoguchi K, Otera H, Mihara K
EMBO J., 2006-11-16;25(24):5635-47.
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human HTRA2/Omi Antibody
There are currently no reviews for this product. Be the first to review Human HTRA2/Omi Antibody and earn rewards!
Have you used Human HTRA2/Omi Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image