Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1009727
View all IFN-kappa Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IFN‑ kappa
Leu28-Lys207
Accession # Q90W0

Specificity

Detects human IFN‑ kappa in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human IFN‑ kappa Antibody

Human IFN-? Antibody in ELISA Standard Curve.

Human IFN‑ kappa ELISA Standard Curve.

Recombinant Human IFN-kappa protein was serially diluted 2-fold and captured by Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB10247) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB102472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IFN‑ kappa Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IFN‑ kappa Monoclonal Antibody (Catalog # MAB10247).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-kappa

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Long Name

Interferon kappa

Alternate Names

IFNK, IFNkappa

Entrez Gene IDs

56832 (Human); 387510 (Mouse)

Gene Symbol

IFNK

UniProt

Q90W0

Additional IFN-kappa Products

Product Documents for Human IFN‑ kappa Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IFN‑ kappa Antibody

For research use only

Related Research Areas

Interferons

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