Human Ig Lambda Light Chain Antibody

Catalog #: MAB10049 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10049-100
MAB10049-SP

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of Human IgG Lambda Light Chain by Western Blot.

3 Images

View All Products for Ig Lambda Light Chain

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ig Lambda Light Chain Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Ig Lambda Light Chain in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1000011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Ig Lambda Light Chain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

See below

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100491).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human IgG Lambda Light Chain by Western Blot. Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Lambda Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human Ig Lambda Light Chain by Simple Western^TM. Simple Western lane view shows lysates of human kappa light chain protein (negative control), human lambda light chain protein, and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Lambda Light Chain at approximately 35 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

ELISA

View Larger

Human Ig Lambda Light Chain ELISA Standard Curve. Human Ig Lambda Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100491) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ig Lambda Light Chain

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain. Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Long Name

Immunoglobulin Lambda Light Chain

Alternate Names

Bence Jones protein; BJP; Constant region of lambda light chains; HCAK 1; HCAK1; Ig kappa chain C region; Ig lambda chain C regions; Ig Lambda Light Chain; IGKC; IGLC 1; IGLC 2; IGLC 3; IGLC; IGLC1; IGLC2; IGLC3; Immunoglobulin kappa constant region; Immunoglobulin kappa constant; Immunoglobulin kappa light chain; Immunoglobulin lambda constant 1; Kappa 1 immunoglobulin light chain; Km; Mcg marker; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; Paraprotein