Detects human IGFL-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IGF‑I, rhIGF-II, rhIGFL-1, rhIGFL-4, and recombinant mouse IGFL-3 is observed.
Monoclonal Mouse IgG2B Clone # 536825
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IGFL-3 Thr25-Pro125 Accession # Q6UXB1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IGFL-3 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGFL-3 Monoclonal Antibody (Catalog # MAB5970) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGFL-3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IGFL-3 (insulin-like growth factor-like 3) is one of four small (~11 kDa), probably secreted human IGFL family members. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family. Mature IGFL-3 is a 101 amino acid (aa) protein that shares 41% aa identity with the sole mouse IGFL. RT-PCR data indicates its expression within the cerebellum.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.