Detection of Human IgM by Western Blot.
Western blot shows lysates of human serum, monkey serum, mouse serum and rat plasma. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgM at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IgM by Simple WesternTM.
Simple Western lane view shows lysates of human serum, loaded at 0.2 mg/mL. A specific band was detected for IgM at approximately 100 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human IgM ELISA Standard Curve.
Human IgM protein was serially diluted 2-fold and captured by Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB94351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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