Human IgM Antibody Summary
This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IgM by Western Blot. Western blot shows lysates of human serum, monkey serum (negative control), mouse serum (negative control), and rat serum (negative control). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB94351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgM at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IgM by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:4000 dilution. A specific band was detected for IgM at approximately 103 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB94351). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human IgM ELISA Standard Curve. Recombinant Human IgM protein was serially diluted 2-fold and captured by Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB94351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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