Detects human IkB-epsilon in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IkB-epsilon is observed and less than 1% cross-reactivity with recombinant human IkB-beta is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human IkB-epsilon Ser141-Asp500 Accession # O00221
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
IkB‑ epsilon in Human Astrocytoma.
IkB‑ epsilon was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human IkB‑ epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4300) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.
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