Human IkB-epsilon Antibody

Catalog # Availability Size / Price Qty
AF4300
AF4300-SP
IkB‑ epsilon  in Human Astrocytoma.
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Human IkB-epsilon Antibody Summary

Species Reactivity
Human
Specificity
Detects human IkB-epsilon in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IkB-epsilon is observed and less than 1% cross-reactivity with recombinant human IkB-beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IkB-epsilon
Ser141-Asp500
Accession # O00221
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry IkB-e antibody in Human Astrocytoma by Immunohistochemistry (IHC-P). View Larger

IkB‑ epsilon in Human Astrocytoma. IkB-e was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human IkB-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4300) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-epsilon

NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Long Name
I-kappa-B-epsilon
Entrez Gene IDs
4794 (Human); 18037 (Mouse)
Alternate Names
epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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