Detects human IkB-epsilon in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IkB-epsilon is observed and less than 1% cross-reactivity with recombinant human IkB-beta is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human IkB-epsilon Ser141-Asp500 Accession # O00221
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IkB‑ epsilon in Human Astrocytoma. IkB‑ epsilon was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human IkB‑ epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4300) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.
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