Human IKK epsilon Antibody

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Detection of Human IKK epsilon  by Western Blot.
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Product Details
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Human IKK epsilon Antibody Summary

Species Reactivity
Detects human IKK epsilon in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human IKK epsilon
Accession # Q14164
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
10-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IKKe antibody by Western Blot. View Larger

Detection of Human IKK epsilon by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IKKe Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IKKe at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IKKe antibody in Ramos Human Cell Line by Immunocytochemistry (ICC). View Larger

IKK epsilon in Ramos Human Cell Line. IKKe was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Goat Anti-Human IKKe Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK epsilon

I kappa B kinase epsilon (IKK epsilon ) is also known as IKBKE and IKKi. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK epsilon is an IKK-related kinase with 30% structural homology to IKK alpha and beta, and is present in a subset of IKK complexes. IKK epsilon activation has been implicated in the initiation of anti-viral gene expression and in the pathogenesis of breast cancer.

Long Name
IkB Kinase epsilon
Entrez Gene IDs
9641 (Human); 56489 (Mouse)
Alternate Names
EC 2.7.11; EC; I-kappa-B kinase epsilon; IKBKE; IKK epsilon; IKK-E; IKKEMGC125297; IKK-epsilon; IKKI; IKK-I; IKK-iMGC125295; Inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon; KIAA0151IKK-related kinase epsilon; MGC125294

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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