Human IL-9 Antibody

Recombinant Monoclonal Antibody.
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-9 in Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1043G
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
    Gln19-Ile144
    Accession # P15248
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IL‑9 by Western Blot. Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), and Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF‑ beta 1 (Catalog # 240-B). PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human IL‑9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL‑9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    • 12 months from date of receipt, -20 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 °C under sterile conditions after opening.
Background: IL-9
Interleukin-9 (IL-9) is a heavily glycosylated cytokine with a 14 kDa peptide backbone that is secreted by CD4+ Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.
  • Long Name:
    Interleukin 9
  • Entrez Gene IDs:
    3578 (Human); 16198 (Mouse)
  • Alternate Names:
    Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunoprecipitation.

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 IP Human Anonymous 10/09/2017
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Summary

ApplicationImmunoprecipitation
Sample TestedHuman recombinant antibody
SpeciesHuman

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