Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1043G
View all IL-9 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
Gln19-Ile144
Accession # P15248

Specificity

Detects human IL-9 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human IL‑9 Antibody

Detection of Human IL-9 antibody by Western Blot.

Detection of Human IL‑9 by Western Blot.

Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), and Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF-beta 1 (Catalog # 240-B). PVDF membrane was probed with a 0.1 µg/mL of Rabbit Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IL‑9 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), and Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF‑ beta 1 (Catalog # 240-B)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2091 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-9

Interleukin-9 (IL-9) is a heavily glycosylated cytokine with a 14 kDa peptide backbone that is secreted by CD4+ Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.

Long Name

Interleukin 9

Alternate Names

IL9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Gene Symbol

IL9

UniProt

P15248

Additional IL-9 Products

Product Documents for Human IL‑9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑9 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Customer Reviews for Human IL‑9 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
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2 Stars
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1 Stars
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  • Human IL-9 Antibody
    Name: Alisha Freeman
    Application: ELISA
    Sample Tested: 4T1.2 mouse mammary carcinoma cells
    Species: Mouse
    Verified Customer | Posted 04/05/2018
  • Human IL-9 Antibody
    Name: Alisha Freeman
    Application: Immunoprecipitation
    Sample Tested: Human recombinant antibody
    Species: Human
    Verified Customer | Posted 10/09/2017

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