Detection of Human IL‑9 by Western Blot.
Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), and Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF‑ beta 1 (Catalog # 240-B). PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human IL‑9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL‑9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
12 months from date of receipt, -20 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 °C under sterile conditions after opening.
Interleukin-9 (IL-9) is a heavily glycosylated cytokine with a 14 kDa peptide backbone that is secreted by CD4+ Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.
Entrez Gene IDs:
3578 (Human); 16198 (Mouse)
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40
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We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 applications: ELISA, Immunoprecipitation.