Human IFN-gamma Antibody

Catalog # Availability Size / Price Qty
MAB285-SP
MAB285-100
MAB285-500
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IFN‑ gamma  in Human PBMCs.
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Product Details
Citations (27)
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Reviews (4)

Human IFN-gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma.
Source
Monoclonal Mouse IgG2A Clone # 25718
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IFN-gamma
Gln24-Gln166
Accession # AAP20098.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 1 ng/mL Recombinant Human IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IFN‑? antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IFN‑ gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry Detection of IFN- gamma antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with either (A) Mouse Anti-Human IFN- gamma Monoclonal Antibody (Catalog # MAB285) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Neutralization IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma  Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN-gamma Monoclonal Antibody (Catalog # MAB285). The ND50 is typically 0.02-0.06 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Citations for Human IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Dissecting human population variation in single-cell responses to SARS-CoV-2
    Authors: Aquino, Y;Bisiaux, A;Li, Z;O'Neill, M;Mendoza-Revilla, J;Merkling, SH;Kerner, G;Hasan, M;Libri, V;Bondet, V;Smith, N;de Cevins, C;Ménager, M;Luca, F;Pique-Regi, R;Barba-Spaeth, G;Pietropaoli, S;Schwartz, O;Leroux-Roels, G;Lee, CK;Leung, K;Wu, JT;Peiris, M;Bruzzone, R;Abel, L;Casanova, JL;Valkenburg, SA;Duffy, D;Patin, E;Rotival, M;Quintana-Murci, L;
    Nature
    Species:  Human
    Sample Types: Cell Culture Superantes
    Applications: Luminex (Detection)
  2. A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors
    Authors: King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ;
    Cancer immunology research
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata
    Authors: R Laufer Bri, A Keren, M Bertolini, Y Ullmann, R Paus, A Gilhar
    Elife, 2023-03-17;12(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor
    Authors: AKO Rode, TB Buus, V Mraz, FAH Al-Jaberi, DV Lopez, SL Ford, S Hennen, IP Eliasen, IV Klewe, L Gharehdagh, A Dragan, MM Rosenkilde, A Woetmann, L Skov, N Ødum, CM Bonefeld, M Kongsbak-W, C Geisler
    Cells, 2022-08-19;11(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  5. Distinct systemic and mucosal immune responses during acute SARS-CoV-2 infection
    Authors: N Smith, P Goncalves, B Charbit, L Grzelak, M Beretta, C Planchais, T Bruel, V Rouilly, V Bondet, J Hadjadj, N Yatim, H Pere, SH Merkling, A Ghozlane, S Kernéis, F Rieux-Lauc, B Terrier, O Schwartz, H Mouquet, D Duffy, JP Di Santo
    Nature Immunology, 2021-09-01;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  6. Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway
    Authors: F Ma, H Hao, X Gao, Y Cai, J Zhou, P Liang, J Lv, Q He, C Shi, D Hu, B Chen, L Zhu, X Xiao, S Li
    Theranostics, 2020-06-19;10(17):7730-7746.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. In the Absence of a TCR Signal IL-2/IL-12/18-Stimulated gammadelta T Cells Demonstrate Potent Anti-Tumoral Function Through Direct Killing and Senescence Induction in Cancer Cells
    Authors: K Schilbach, C Welker, N Krickeberg, C Kai beta er, S Schleicher, H Hashimoto
    Cancers (Basel), 2020-01-04;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease
    Authors: PA Szabo, HM Levitin, M Miron, ME Snyder, T Senda, J Yuan, YL Cheng, EC Bush, P Dogra, P Thapa, DL Farber, PA Sims
    Nat Commun, 2019-10-17;10(1):4706.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. DDIT4 and Associated lncDDIT4 Modulate Th17 Differentiation through the DDIT4/TSC/mTOR Pathway
    Authors: F Zhang, G Liu, D Li, C Wei, J Hao
    J. Immunol., 2018-01-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  10. PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2
    Authors: Y Liu, L Qu, Y Liu, B Roizman, GG Zhou
    Proc. Natl. Acad. Sci. U.S.A., 2017-07-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Bilirubin suppresses Th17 immunity in colitis by upregulating CD39
    Authors: MS Longhi, M Vuerich, A Kalbasi, JE Kenison, A Yeste, E Csizmadia, B Vaughn, L Feldbrugge, S Mitsuhashi, B Wegiel, L Otterbein, A Moss, FJ Quintana, SC Robson
    JCI Insight, 2017-05-04;2(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion
    J Immunol, 2016-08-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  13. Modulation of Stat-1 in Human Macrophages Infected with Different Species of Intracellular Pathogenic Bacteria
    J Immunol Res, 2016-06-29;2016(0):5086928.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Production of Proinflammatory Cytokines by Monocytes in Liver-Transplanted Recipients with De Novo Autoimmune Hepatitis Is Enhanced and Induces TH1-like Regulatory T Cells
    Authors: AS Arterbery, A Osafo-Addo, Y Avitzur, M Ciarleglio, Y Deng, SJ Lobritto, M Martinez, DA Hafler, M Kleinewiet, UD Ekong
    J. Immunol., 2016-04-18;196(10):4040-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
    Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil
    Nat Immunol, 2015-01-26;16(3):318-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
    Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
    PLoS ONE, 2014-05-02;9(5):e96695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation.
    Authors: Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, McKinley C, Ahyi AN, Han L, Nguyen ET, Robertson MJ, Perumal NB, Tepper RS, Nutt SL, Kaplan MH
    Nat. Immunol., 2010-05-02;11(6):527-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Impaired development of human Th1 cells in patients with deficient expression of STAT4.
    Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH
    Blood, 2009-04-09;113(23):5887-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  19. In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
    Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
    J. Clin. Invest., 2007-12-01;117(12):3868-3878.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
    Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
    Exp. Hematol., 2006-10-01;34(10):1312-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Peripheral expansion of circulating T-helper 1 cells predicts coronary endothelial dysfunction after cardiac transplantation.
    Authors: Methe H, Wiegand D, Welsch U, Meiser B, Nabauer M, Koglin J
    J. Heart Lung Transplant., 2005-07-01;24(7):833-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  23. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004-07-15;173(2):1463-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Reprogramming of IL-10 activity and signaling by IFN-gamma.
    Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
    J. Immunol., 2003-11-15;171(10):5034-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
    Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y
    Blood, 2003-07-31;102(12):4084-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Cutting edge: immune cells as sources and targets of the IL-10 family members?
    Authors: Wolk K, Kunz S, Asadullah K, Sabat R
    J. Immunol., 2002-06-01;168(11):5397-402.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  27. Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
    Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
    J. Immunol., 2002-01-01;168(1):505-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human IFN-gamma Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human IFN-gamma Antibody
By Anonymous on 06/17/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Human IFN-gamma Antibody
By Anonymous on 04/28/2021
Application: B/N Sample Tested: Cell Culture Media Species: Human

Human IFN-gamma Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human IFN-gamma Antibody
By Carly Garrison on 01/09/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human