Human Integrin beta 1/CD29 Antibody

Name: Human Integrin beta 1/CD29 Antibody
Brand: R&D Systems
SKU: MAB1778
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB1778

Submit a review

Citations (14)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4B7R

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Ocular melanoma cell line V+B2

Specificity

Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin beta 1/CD29 Antibody

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Integrin beta 1/CD29 in MG‑63 Human Cell Line.

Integrin beta 1/CD29 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Anti-body (Catalog # MAB1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Anti-body (yellow; NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Integrin beta 1/CD29 in A549 Cells.

Integrin beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

Applications for Human Integrin beta 1/CD29 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line, A549 human lung carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin cancer tissue

Immunoprecipitation

Marshall, J.F. et al. (1998) Br. J. Cancer 77:522.

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin beta 1/CD29 under non-reducing conditions only

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)