Human Kallikrein 3/PSA Antibody
Human Kallikrein 3/PSA Antibody Summary
Accession # NP_001639
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Kallikrein 3/PSA in LNCaP and MCF-7 Human Cell Lines. Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13441) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Kallikrein 3/PSA in Human Prostate. Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13441) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 3/PSA
Kallikrein 3 is a member of the Kallikrein family of serine proteases. It is also known as PSA (prostate-specific antigen) and is used in prostate cancer diagnosis.
Citation for Human Kallikrein 3/PSA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Transient induction of ING4 by Myc drives prostate epithelial cell differentiation and its disruption drives prostate tumorigenesis.
Authors: Berger P, Frank S, Schulz V, Nollet E, Edick M, Holly B, Chang T, Hostetter G, Kim S, Miranti C
Cancer Res, 2014-04-24;74(12):3357-68.
Sample Types: Whole Cells
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Kallikrein 3/PSA Antibody
There are currently no reviews for this product. Be the first to review Human Kallikrein 3/PSA Antibody and earn rewards!
Have you used Human Kallikrein 3/PSA Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image