Human Kallikrein 3/PSA Antibody
Human Kallikrein 3/PSA Antibody Summary
Accession # P07288
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Kallikrein 3/PSA by Western Blot. Western blot shows lysates of human prostate tissue, human prostate cancer tissue, and DU145 human prostate carcinoma cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13443) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kallikrein 3/PSA at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kallikrein 3/PSA in LNCaP Human Cell Line. Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13443) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Kallikrein 3/PSA in Human Prostate. Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13443) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 3/PSA
Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5-2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer. The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). Recombinant human PSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha 1-antichymotrypsin (ACT) and alpha 2-macroglobulin are known to form complexes with PSA.
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
- Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
- Lilja H. (2003) Urology 62:270.
- Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.
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