Human Kallikrein 4/Prostase/EMSP1 Antibody

Catalog # Availability Size / Price Qty
MAB35661
MAB35661-SP
Kallikrein 4/Prostase/EMSP1 in Human Prostate.
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Human Kallikrein 4/Prostase/EMSP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 4/Prostase/EMSP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Kallikrein 1, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, rhKallikrein B1, rhFactor VII, rhFactor X, rhFactor XI, rhHGFA, rhThrombin, and rhUPA-1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 325712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 4/Prostase/EMSP1
Ser27-Ser254
Accession # NP_004908
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Kallikrein 4/Prostase/EMSP1 (Catalog # 1719-SE) under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 4/Prostase/
EMSP1 (Catalog # 1719-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 4/Prostase/EMSP1 (1.0 µg/mL, Catalog # 1719‑SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 2.9 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Kallikrein 4/Prostase/EMSP1 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

Kallikrein 4/Prostase/EMSP1 in Human Prostate. Kallikrein 4/Prostase/EMSP1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 4/Prostase/EMSP1 Monoclonal Antibody (Catalog # MAB35661) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 4/Prostase/EMSP1

Kallikrein 4 (KLK4), also known as prostase or EMSP1 (enamel matrix serine protease 1), is a serine protease of the human tissue kallikrein gene family (1). Among normal tissues, human KLK4 is specifically expressed in the prostate (2). It is over-expressed in prostate cancer and this expression is regulated by hormones including androgens, esterogen and progesterone (3). Recombinant human KLK4 readily activates pro-KLK3/PSA and pro-urokinase type plasminogen activator (uPA), indicating it may initiate events involving PSA and uPA in either normal or abnormal processes (4). KLK4 may have additional roles such as functioning as one of the two major enamel proteases identified that process enamel matrix proteins (5). In addition to being a secreted enzyme, it is also a nuclear protein (3, 6). The deduced amino acid sequence of human KLK4 consists of a signal peptide, a short pro region and a mature/active enzyme.

References
  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Nelson, P.S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3114.
  3. Xi, Z. et al. (2004) Cancer Res. 64:2365.
  4. Takayama, T.K. et al. (2001) Biochemistry 40:15341.
  5. Simmer, J.P. and J.C. Hu (2002) Connect Tissue Res. 43:441.
  6. Ryu, O.H. et al. (2002) Eur. J. Oral. Sci. 110:358.
Entrez Gene IDs
9622 (Human)
Alternate Names
AI2A1; ARM1; EC 3.4.21; EC 3.4.21.-; EMSP; EMSP1; EMSP1MGC116827; Enamel matrix serine proteinase 1; kallikrein 4 (prostase, enamel matrix, prostate); Kallikrein 4; kallikrein-4; Kallikrein-like protein 1; kallikrein-related peptidase 4; KLK4; KLK-L1MGC116828; Prostase; PRSS17enamel matrix serine protease 1; PSTSandrogen-regulated message 1; Serine protease 17

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