|Katanin p60 in Human Brain. Katanin p60 was detected in immersion fixed paraffin-embedded sections of human brain (globus pallidus) using Mouse Anti-Human Katanin p60 Monoclonal Antibody (Catalog # MAB7100) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
KATNA1 (Katanin [Japanese word for sword] p60 ATPase-containing subunit A1; also Katanin p60) is a 55-60 kDa member of the VPS4 subfamily, AAA ATPase family of molecules. It is ubiquitously expressed and is recognized to sever microtubules, allowing for their reorganization during cell division and migration. KATNA1 is most effective on nonacetylated, non-tau binding microtubules. In general, KATNA1 activity is regulated by binding to KATNB1/katanin p80, which potentiates KATNA1 action. However, the relationship is complex, and governed by the local p60:p80 ratio. Human KATNA1 is 491 amino acids (aa) in length. It contains a p80 interaction segment (aa 1-29), followed by a microtubule interaction region (aa 30-185), an ATPase domain (aa 239-381), and an oligomerization region (aa 455-489). KATNA1 is phosphorylated on Ser170. There is one isoform variant that shows a deletion of aa 168-243 coupled to a four aa substitution for aa 384-491. Over aa 308-378, human KATNA1 shares 97% aa identity with mouse KATNA1.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided