Human Laminin alpha 2 Antibody

Catalog # Availability Size / Price Qty
MAB2143-100
MAB2143-SP
Laminin  alpha 2 in Human Heart.
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Human Laminin alpha 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Laminin alpha 2 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1003531
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human Laminin alpha 2
Lys2145-Asn3122
Accession # P24043
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Laminin  alpha 2 in Human Heart. View Larger

Laminin alpha 2 in Human Heart. Laminin alpha 2 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry Laminin  alpha 2 in U2OS and MCF-7 Human Cell Lines. View Larger

Laminin alpha 2 in U2OS and MCF-7 Human Cell Lines. Laminin alpha 2 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and MCF-7 human breast cancer cell line (negative stain) using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 2

Laminin subunit alpha-2 (LAMA-2) is an extracellular matrix protein that is a main component of the basement membrane. It is thought to mediate the attachment, migration and organization of cells during embryonic development via interacting with other components with the extracellular matrix. It is widely expressed except in the liver, thymus and bone.

Entrez Gene IDs
3908 (Human); 16773 (Mouse); 309368 (Rat)
Alternate Names
LAMA2; Laminin alpha 2; Laminin M chain; laminin subunit alpha-2; laminin, alpha 2; Laminin-12 subunit alpha; Laminin-2 subunit alpha; LAMM; LAMMLaminin-4 subunit alpha; Merosin heavy chain; Merosin

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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