Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1003531
View all Laminin alpha 2 Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Laminin alpha 2
Lys2145-Asn3122
Accession # P24043

Specificity

Detects human Laminin alpha 2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Laminin alpha 2 Antibody

Laminin alpha 2 antibody in Human Heart by Immunohistochemistry (IHC-P).

Laminin alpha 2 in Human Heart.

Laminin alpha 2 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Laminin alpha 2 antibody in U2OS and MCF-7 Human Cell Lines by Immunocytochemistry (ICC).

Laminin alpha 2 in U2OS and MCF-7 Human Cell Lines.

Laminin alpha 2 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and MCF-7 human breast cancer cell line (negative stain) using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Laminin alpha 2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line and MCF-7 human breast cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Reviewed Applications

Read 1 review rated 5 using MAB2143 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin alpha 2

Laminin subunit alpha-2 (LAMA-2) is an extracellular matrix protein that is a main component of the basement membrane. It is thought to mediate the attachment, migration and organization of cells during embryonic development via interacting with other components with the extracellular matrix. It is widely expressed except in the liver, thymus and bone.

Alternate Names

LAMA2, LAMM, Merosin

Entrez Gene IDs

3908 (Human); 16773 (Mouse); 309368 (Rat)

Gene Symbol

LAMA2

UniProt

P24043

Additional Laminin alpha 2 Products

Product Documents for Human Laminin alpha 2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Laminin alpha 2 Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Customer Reviews for Human Laminin alpha 2 Antibody (1)

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  • Human Laminin alpha 2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Heart tissue
    Species: Human
    Verified Customer | Posted 10/22/2022
    Human Laminin alpha 2 Antibody MAB2143

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Protocols

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