Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 546215
View all Laminin alpha 3/Laminin-5 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Laminin alpha 3/Laminin‑5
aa 21-1713
Accession # NP_000218

Specificity

Detects human Laminin alpha 3/Laminin‑5 in direct ELISAs and Western blots. In direct ELISAs, no cross reactivity with recombinant human (rh) Laminin  alpha 1, beta 1, gamma 2, or recombinant mouse Laminin  alpha 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Laminin  alpha 3/Laminin‑5 Antibody

Detection of Human Laminin a3/Laminin-5 antibody by Western Blot.

Detection of Human Laminin alpha 3/Laminin‑5 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Laminin a3/ Laminin-5 Monoclonal Antibody (Catalog # MAB21441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Laminin a3/Laminin-5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Laminin a3/Laminin-5 antibody in NHEK Human Cell Line by Immunocytochemistry (ICC).

Laminin alpha 3/Laminin‑5 in NHEK Human Cell Line.

Laminin a3/Laminin-5 was detected in immersion fixed NHEK human normal epidermal keratinocytes treated with 10ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 24 hours using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB21441) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear space. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Laminin a3/Laminin-5 antibody in Human Stomach by Immunohistochemistry (IHC-P).

Laminin alpha 3/Laminin‑5 in Human Stomach.

Laminin a3/Laminin-5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB21441) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Laminin a3/Laminin-5 antibody in U2OS Human Cell Line antibody by Flow Cytometry.

Detection of Laminin alpha 3/Laminin-5 in U2OS Human Cell Line by Flow Cytometry.

U2OS human osteosarcoma cell line was stained with Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB21441, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.

Applications for Human Laminin  alpha 3/Laminin‑5 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NHEK human normal epidermal keratinocytes treated with Recombinant Human TGF‑ beta 1 (Catalog # 240-B)

Immunohistochemistry

5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Laminin alpha 3, see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: U2OS Human cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

2 µg/mL
Sample: Human lung tissue under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 5 using MAB21441 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma  chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival. Laminin alpha 3/Laminin-5, also known as epiligrin, includes alpha 3, beta 3, and gamma 2 subunits. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and contributes to initiation and maintenance of epithelial cell anchorage to the underlying connective tissue. Within aa 21‑1713 of the alpha 3 subunit, human and mouse share 77% amino acid sequence identity.

Alternate Names

Epiligrin, LAMA3, Laminin-5, Laminin5

Entrez Gene IDs

3909 (Human)

Gene Symbol

LAMA3

UniProt

NP_000218

Additional Laminin alpha 3/Laminin-5 Products

Product Documents for Human Laminin  alpha 3/Laminin‑5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Laminin  alpha 3/Laminin‑5 Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human Laminin  alpha 3/Laminin‑5 Antibody

Customer Reviews for Human Laminin  alpha 3/Laminin‑5 Antibody (3)

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3 Customer Ratings
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  • Human Laminin  alpha 3/Laminin-5 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Stomach tissue
    Species: Human
    Verified Customer | Posted 08/29/2022
    Human Laminin  alpha 3/Laminin‑5 Antibody MAB21441
  • Human Laminin  alpha 3/Laminin-5 Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 11/09/2021
    Human Laminin  alpha 3/Laminin‑5 Antibody MAB21441
  • Human Laminin  alpha 3/Laminin-5 Antibody
    Name: Hendri Pas
    Application: Immunohistochemistry frozen
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 09/07/2018
    Immunofluorescent staining of frozen human skin.
    Human Laminin  alpha 3/Laminin‑5 Antibody MAB21441

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Protocols

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