Human LCoR Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LCoR in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 731229
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human LCoR
    Asn110-Gln338
    Accession # Q96JN0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
LCoR in MCF‑7 Human Cell Line. LCoR was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human LCoR Monoclonal Antibody (Catalog # MAB7534) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LCoR
LCoR (ligand-dependent corepressor), also known as MLR2 (Mblk1-related protein 2), is a 47-50 kDa nuclear phosphoprotein that is a corepressor of ligand-dependent transcriptional activation by nuclear receptors such as estrogen and progesterone receptors. It is widely expressed throughout fetal development and in the adult. The 433 amino acid (aa) LCoR contains two N-terminal PxDLS nuclear receptor interaction motifs, a central HDAC interaction motif, a nuclear localization signal, and a C‑terminal Helix-Turn-Helix motif. Alternate splicing produces a truncated 406 aa variant. Within the region used as an immunogen, human LCoR shares 97% aa identity with mouse and rat LCoR.
  • Long Name:
    Ligand-dependent Nuclear Receptor Corepressor
  • Entrez Gene IDs:
    84458 (Human); 212391 (Mouse); 365462 (Rat)
  • Alternate Names:
    FLJ38026; KIAA1795RP11-175O19.1; LCoR; ligand dependent nuclear receptor corepressor; Mblk1-related protein 2; MLR2; MLR2ligand-dependent corepressor
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