Human LCoR Antibody

Catalog # Availability Size / Price Qty
MAB7534
MAB7534-SP
LCoR in MCF‑7 Human Cell Line.
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Human LCoR Antibody Summary

Species Reactivity
Human
Specificity
Detects human LCoR in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 731229
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human LCoR
Asn110-Gln338
Accession # Q96JN0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry LCoR antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

LCoR in MCF‑7 Human Cell Line. LCoR was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human LCoR Monoclonal Antibody (Catalog # MAB7534) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LCoR

LCoR (ligand-dependent corepressor), also known as MLR2 (Mblk1-related protein 2), is a 47-50 kDa nuclear phosphoprotein that is a corepressor of ligand-dependent transcriptional activation by nuclear receptors such as estrogen and progesterone receptors. It is widely expressed throughout fetal development and in the adult. The 433 amino acid (aa) LCoR contains two N-terminal PxDLS nuclear receptor interaction motifs, a central HDAC interaction motif, a nuclear localization signal, and a C‑terminal Helix-Turn-Helix motif. Alternate splicing produces a truncated 406 aa variant. Within the region used as an immunogen, human LCoR shares 97% aa identity with mouse and rat LCoR.

Long Name
Ligand-dependent Nuclear Receptor Corepressor
Entrez Gene IDs
84458 (Human); 212391 (Mouse); 365462 (Rat)
Alternate Names
FLJ38026; KIAA1795RP11-175O19.1; LCoR; ligand dependent nuclear receptor corepressor; Mblk1-related protein 2; MLR2; MLR2ligand-dependent corepressor

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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