Detection of Human LEDGF by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human CD4+ T cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEDGF p75 at approximately 75 kDa andLEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LEDGF in HeLa Human Cell Line. LEDGF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LEDGF, also known as transcription co-activator p75, is a 75 kDa
protein that belongs to the hepatoma-derived growth factor (HDGF)
family of nuclear targeted proteins. It is a widely expressed protein
that interacts with lentiviral integrase. As a result of alternative
splicing, isoforms with deletions, substitutions/insertions have been
reported. Human LEDGF shares 91%, 92% and 96% amino acid sequence
homology with rat, mouse and feline LEDGF, respectively.
Lens Epithelium-derived Growth Factor
Entrez Gene IDs:
11168 (Human); 101739 (Mouse); 313323 (Rat)
CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; DFS70; LEDGFDFS 70; Lens epithelium-derived growth factor; MGC74712; p52; p75; PAIP; PC4 and SFRS1 interacting protein 1; PC4 and SFRS1 interacting protein 2; PC4 and SFRS1-interacting protein; PSIP1; PSIP2; transcriptional coactivator p52/p75; Transcriptional coactivator p75/p52
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