Detection of Recombinant Human Leptin R by Western Blot.
Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR), Recombinant Mouse Leptin R Fc Chimera (Catalog # 7814-LR) and Recombinant Rat Leptin R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin R at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Leptin R in Human Brain.
Leptin R was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin R
Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and potentially encoding a soluble form of the receptor, has also been described. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.
Human OB-R long form encodes a 1165 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 819 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 303 aa residue cytoplasmic domain. The extracellular domain of OB-R contain two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.
Tartaglia, L.A. et al. (1995) Cell 83:1263.
Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
Entrez Gene IDs:
3953 (Human); 16847 (Mouse)
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295
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