Human Leptin R Antibody

Catalog # Availability Size / Price Qty
AF389
AF389-SP
Detection  of Recombinant Human Leptin R by Western Blot.
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Product Details
Citations (2)
FAQs
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Human Leptin R Antibody Summary

Species Reactivity
Human
Specificity
Detects human Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse Leptin R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Leptin R
Thr20-Asp839
Accession # P48357
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
15-30 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Recombinant Human Leptin R by Western Blot. View Larger

Detection of Recombinant Human Leptin R by Western Blot. Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR), Recombinant Mouse Leptin R Fc Chimera (Catalog # 7814-LR) and Recombinant Rat Leptin R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin R at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry Leptin R in Human Brain. View Larger

Leptin R in Human Brain. Leptin R was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and potentially encoding a soluble form of the receptor, has also been described. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Human OB-R long form encodes a 1165 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 819 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 303 aa residue cytoplasmic domain. The extracellular domain of OB-R contain two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.

References
  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
Long Name
Leptin Receptor
Entrez Gene IDs
3953 (Human); 16847 (Mouse)
Alternate Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295

Product Datasheets

Citations for Human Leptin R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Leptin promotes wound healing in the skin.
    Authors: Tadokoro, Susumu, Ide, Shinji, Tokuyama, Reiko, Umeki, Hirochik, Tatehara, Seiko, Kataoka, Shiki, Satomura, Kazuhito
    PLoS ONE, 2015;10(3):e0121242.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Embryonic adhesion is not affected by endometrial leptin receptor gene silencing.
    Authors: Cervero A, Dominguez F, Horcajadas JA, Quinonero A, Pellicer A, Simon C
    Fertil. Steril., 2007;88(4):1086-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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