Human Leptin R Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse Leptin R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Leptin R
    Thr20-Asp839
    Accession # P48357
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    15-30 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human Leptin R by Western Blot. Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR), Recombinant Mouse Leptin R Fc Chimera (Catalog # 7814-LR) and Recombinant Rat Leptin R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin R at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
Leptin R in Human Brain. Leptin R was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF389) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and potentially encoding a soluble form of the receptor, has also been described. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Human OB-R long form encodes a 1165 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 819 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 303 aa residue cytoplasmic domain. The extracellular domain of OB-R contain two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.

  • References:
    1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
    2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
    3. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
  • Long Name:
    Leptin Receptor
  • Entrez Gene IDs:
    3953 (Human); 16847 (Mouse)
  • Alternate Names:
    B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Leptin promotes wound healing in the skin.
    Authors: Tadokoro, Susumu, Ide, Shinji, Tokuyama, Reiko, Umeki, Hirochik, Tatehara, Seiko, Kataoka, Shiki, Satomura, Kazuhito
    PLoS ONE, 2015;10(3):e0121242.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Embryonic adhesion is not affected by endometrial leptin receptor gene silencing.
    Authors: Cervero A, Dominguez F, Horcajadas JA, Quinonero A, Pellicer A, Simon C
    Fertil. Steril., 2007;88(4):1086-92.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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NL996
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VCTS022
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VCTS021
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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