Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LITAF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LITAF
Met1-Ala111
Accession # Q99732

Specificity

Detects human LITAF in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LITAF Antibody

Detection of Human LITAF antibody by Western Blot.

Detection of Human LITAF by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LITAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4695) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LITAF at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LITAF antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

LITAF in A431 Human Cell Line.

LITAF was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human LITAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4695) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LITAF antibody in Human Small Intestine by Immunohistochemistry (IHC-P).

LITAF in Human Small Intestine.

LITAF was detected in immersion fixed paraffin-embedded sections of human small intestine using Goat Anti-Human LITAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4695) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lymphocytes in lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human LITAF Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LITAF

LITAF (LPS-induced TNF- alpha factor), initially identified through its interaction with the TNF-alpha promoter, is a transcription factor that contributes to the regulation of several inflammatory cytokines in response to LPS or p53 stimulation. LITAF interacts directly with LPS-induced STAT6(B) in the cytoplasm, this complex then translocates into the nucleus, where it significantly up-regulates the transcription of other inflammatory mediators such as, GRO, IL-1 alpha, TNF-alpha, MCP-2 and IFN-gamma. Phosphorylation of LITAF by p38 alpha via the TLR pathway is also required for nuclear translocation. Mutations in LITAF have been associated with CMT1C
(Charcot‑Marie-Tooth neuropathy type 1C) an autosomal dominant demyelinating form of peripheral neuropathy.

Long Name

Lipopolysaccharide-induced TNF Factor

Alternate Names

PIG7, SIMPLE, TP53I7

Entrez Gene IDs

9516 (Human); 56722 (Mouse); 65161 (Rat)

Gene Symbol

LITAF

UniProt

Q99732

Additional LITAF Products

Product Documents for Human LITAF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LITAF Antibody

For research use only

Citations for Human LITAF Antibody

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Protocols

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