Human LRP-5 Intracellular Domain Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRP-5 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with the intracellular domain of recombinant human LRP-6 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 747619
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human LRP-5 Intracellular Domain
    Phe1422-Ser1615
    Accession # O75197
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human LRP-5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-5 Intracellular Domain Monoclonal Antibody (Catalog # MAB6646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-5 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRP-5

LRP-5 (low-density lipoprotein receptor-related protein 5) is a 1615 amino acid (aa), ~180‑200 kDa type I transmembrane glycoprotein. The 1353 aa extracellular domain (ECD) contains 20 class B LDLR, 4 EGF‑like, and 3 class A LDLR repeats. Human LRP-5 aa 745-1097 encompasses class B LDLR repeats #12-17 and EGF‑like repeat #3. It shares 94% aa identity with mouse and rat LRP-5, and 70% aa identity with human LRP-6 within corresponding regions. LRP-5 and LRP-6 mediate DKK effects on Wnt signaling. LRP-5 polymorphisms correlate with susceptibility and resistance to osteoporosis.

  • Long Name:
    LDL Receptor-related Protein 5
  • Entrez Gene IDs:
    4041 (Human); 16973 (Mouse); 293649 (Rat)
  • Alternate Names:
    BMND1; BMND1OPTA1; EVR1; EVR4; HBM; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; low-density lipoprotein receptor-related protein 5; LR3VBCH2; LRP5; LRP-5; LRP7; LRP7exudative vitreoretinopathy 1; OPPG; OPS; OPTA1; osteoporosis pseudoglioma syndrome; VBCH2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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