Detects human LRP-5 Intracellular Domain in direct ELISAs and Western blots.
In direct ELISAs and Western blots, no
cross-reactivity with the intracellular domain of recombinant human LRP-6 is
Monoclonal Mouse IgG2B Clone # 747619
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human LRP-5 Intracellular Domain Phe1422-Ser1615 Accession # O75197
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LRP-5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-5 Intracellular Domain Monoclonal Antibody (Catalog # MAB6646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-5 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRP-5 (low-density lipoprotein receptor-related protein 5) is a 1615 amino acid (aa), ~180‑200 kDa type I transmembrane glycoprotein. The 1353 aa extracellular domain (ECD) contains 20 class B LDLR, 4 EGF‑like, and 3 class A LDLR repeats. Human LRP-5 aa 745-1097 encompasses class B LDLR repeats #12-17 and EGF‑like repeat #3. It shares 94% aa identity with mouse and rat LRP-5, and 70% aa identity with human LRP-6 within corresponding regions. LRP-5 and LRP-6 mediate DKK effects on Wnt signaling. LRP-5 polymorphisms correlate with susceptibility and resistance to osteoporosis.
LDL Receptor-related Protein 5
Entrez Gene IDs:
4041 (Human); 16973 (Mouse); 293649 (Rat)
BMND1; BMND1OPTA1; EVR1; EVR4; HBM; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; low-density lipoprotein receptor-related protein 5; LR3VBCH2; LRP5; LRP-5; LRP7; LRP7exudative vitreoretinopathy 1; OPPG; OPS; OPTA1; osteoporosis pseudoglioma syndrome; VBCH2
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.