LRP-5 (low-density lipoprotein receptor-related protein 5) is a 1615 amino acid (aa), ~180‑200 kDa type I transmembrane glycoprotein. The 1353 aa extracellular domain (ECD) contains 20 class B LDLR, 4 EGF‑like, and 3 class A LDLR repeats. Human LRP-5 aa 745-1097 encompasses class B LDLR repeats #12-17 and EGF‑like repeat #3. It shares 94% aa identity with mouse and rat LRP-5, and 70% aa identity with human LRP-6 within corresponding regions. LRP-5 and LRP-6 mediate DKK effects on Wnt signaling. LRP-5 polymorphisms correlate with susceptibility and resistance to osteoporosis.
Human LRP-5 Intracellular Domain Antibody
R&D Systems | Catalog # MAB6646
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 747619
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Product Specifications
Immunogen
E. coli-derived recombinant human LRP-5 Intracellular Domain
Phe1422-Ser1615
Accession # O75197
Phe1422-Ser1615
Accession # O75197
Specificity
Detects human LRP-5 Intracellular Domain in direct ELISAs and Western blots.
In direct ELISAs and Western blots, no
cross-reactivity with the intracellular domain of recombinant human LRP-6 is
observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LRP-5 Intracellular Domain Antibody
Detection of Human LRP-5 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-5 Intracellular Domain Monoclonal Antibody (Catalog # MAB6646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-5 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human LRP-5 Intracellular Domain Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line
Sample: HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LRP-5
Long Name
LDL Receptor-related Protein 5
Alternate Names
BMND1, EVR1, EVR4, HBM, LRP5, LRP7, OPPG, OPS, OPTA1, VBCH2
Gene Symbol
LRP5
UniProt
Additional LRP-5 Products
Product Documents for Human LRP-5 Intracellular Domain Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human LRP-5 Intracellular Domain Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
