|Lyp in Human Lymph Node. Lyp was detected in immersion fixed paraffin-embedded sections of human lymph node using 25 µg/mL Mouse Anti-Human Lyp Monoclonal Antibody (Catalog # MAB3428) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
|Detection of Human Lyp by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Lyp Monoclonal Antibody (Catalog # MAB3428) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Lyp at approximately 108 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Lymphoid phosphatase (Lyp), also called LyPTP, Protein Tyrosine Phosphatase, Nonreceptor-type 22 (PTPN22), and PEST-domain Phosphatase (PEP), dephosphorylates tyrosine residues in proteins. Found primarily in bone marrow and lymphoid cell lines, an R620W gain-of-function mutation in Lyp has been associated with susceptibility to autoimmune diseases such as type I diabetes, rheumatoid arthritis, and lupus erythematosus. Mature PEP knockout mice have an excess of CD8 positive T cells and an exaggerated antigen-induced proliferative response.
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