Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

Catalog # Availability Size / Price Qty
MAB2639
MAB2639-SP

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Citations (2)
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Human Lysyl Oxidase Homolog 2/LOXL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lysyl Oxidase Homolog 2/LOXL2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 262418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lysyl Oxidase Homolog 2/LOXL2
Gln26-Gln744 (predicted)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Lysyl Oxidase Homolog 2/LOXL2 under non-reducing conditions only (Catalog # 2639-AO)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Lysyl Oxidase Homolog 2/LOXL2 (Catalog # 2639-AO), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysyl Oxidase Homolog 2/LOXL2

Lysyl Oxidase Homolog 2 (lysyl oxidase-like protein 2, LOXL2) is a member of lysyl oxidase-like (LOXL) gene family which includes LOXL1 through LOXL4. These enzymes are secreted copper-binding amine oxidases that oxidize primary amine substrates to aldehydes (1). The N-terminal region of LOXL2 contains four scavenger receptor cysteine-rich (SRCR) domains, and the C-terminal region is a catalytic domain similar to other lysyl oxidases (1). The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone co-factor (2). Although some of the LOXL enzymes are known to cross-link collagen and elastin substrates, such a function has yet to be characterized for LOXL2. It has been shown that LOXL2 promotes cell migration and tumor cell invasiveness (3, 4). Elevated expression of LOXL2 is also associated with cancer progression in various tumors and carcinoma cell lines, which makes it a potential marker for prognosis of cancer (5). LOXL2 is expressed in many tissues, with elevated levels in reproductive tissues such as placenta, uterus, and prostate (6).

References
  1. Csiszar, H. (2001) Prog. Nucleic Acid Res. Mol. Biol. 70:1.
  2. Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381.
  3. Akiri, G. et al. (2003) Cancer Res. 63:1657.
  4. Hollosi, P. et al. (2009) Int. J. Cancer. 125:318.
  5. Peinado, H. et al. (2008) Cancer Res. 68:4541.
  6. Jourdan-Le Saux C. et al. (1999) J. Biol. Chem. 274:12939.
Entrez Gene IDs
4017 (Human); 94352 (Mouse); 102138804 (Cynomolgus Monkey)
Alternate Names
EC 1.4.3; EC 1.4.3.-; LOL2; LOR2; LOXL2; Lysyl Oxidase Homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14; WS9-14

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Citations for Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Bone morphogenetic protein 1.3 inhibition decreases scar formation and supports cardiomyocyte survival after myocardial infarction
    Authors: S Vukicevic, A Colliva, V Kufner, V Martinelli, S Moimas, S Vodret, V Rumenovic, M Milosevic, B Brkljacic, D Delic-Brkl, R Correa, M Giacca, M Maglione, T Bordukalo-, I Dumic-Cule, S Zacchigna
    Nature Communications, 2022;13(1):81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Secreted LOXL2 is a novel therapeutic target that promotes gastric cancer metastasis via the Src/FAK pathway.
    Authors: Peng L, Ran YL, Hu H, Yu L, Liu Q, Zhou Z, Sun YM, Sun LC, Pan J, Sun LX, Zhao P, Yang ZH
    Carcinogenesis, 2009;30(10):1660-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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