Human Lysyl Oxidase Homolog 3/LOXL3 Antibody

Catalog # Availability Size / Price Qty
MAB6069-100
MAB6069-SP
Human  Lysyl Oxidase Homolog 3/LOXL3 ELISA Standard Curve.
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Human Lysyl Oxidase Homolog 3/LOXL3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lysyl Oxidase Homolog 3/LOXL3 protein in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1013328
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived human Lysyl Oxidase Homolog 3/LOXL3 protein
Met1-Ile753
Accession # P58215
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB60691).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Lysyl Oxidase Homolog 3/LOXL3 ELISA Standard Curve. Recombinant Human Lysyl Oxidase Homolog 3/LOXL3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB60691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB6069) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysyl Oxidase Homolog 3/LOXL3

Lysyl oxidase-like protein 3 (LOXL3) is a secreted copper amine oxidase. The N-terminal region of LOXL3 contains four scavenger receptor cysteine-rich (SRCR) domains. The C-terminal region consists of a catalytic domain similar to other lysyl oxidases and a cytokine receptor-like domain (1). The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone cofactor (2). LOXL3 is expressed primarily in epidermis, kidney, aorta, and chondrocytes (3). The enzyme is expressed at low levels in most other tissues. Like lysyl oxidase, LOXL3 oxidizes the lysine epsilon -amino groups of a number of collagen substrates, and is also active against other primary amines (4). However, LOXL3 does not require proteolytic processing to be activated.

References
  1. Csiszar, K. (2001) Prog. Nucl. Acid Res. Mol. Biol. 70:1.
  2. Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381.
  3. Molnar, J. et al. (2003) Biochim. Biophys. Acta 1647:220.
  4. Lee, J. and Y. Kim (2006) J. Biol. Chem. 281:37282.
Entrez Gene IDs
84695 (Human); 16950 (Mouse)
Alternate Names
EC 1.4.3; EC 1.4.3.-; EC 1.4.3.13; LOL3; LOXL; LOXL3; Lysyl Oxidase Homolog 3; lysyl oxidase-like 3; Lysyl oxidase-like protein 3

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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