Human Lysyl Oxidase Homolog 3/LOXL3 Antibody Summary
Accession # P58215
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB60691).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Lysyl Oxidase Homolog 3/LOXL3 ELISA Standard Curve. Recombinant Human Lysyl Oxidase Homolog 3/LOXL3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB60691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Lysyl Oxidase Homolog 3/LOXL3 Monoclonal Antibody (Catalog # MAB6069) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lysyl Oxidase Homolog 3/LOXL3
Lysyl oxidase-like protein 3 (LOXL3) is a secreted copper amine oxidase. The N-terminal region of LOXL3 contains four scavenger receptor cysteine-rich (SRCR) domains. The C-terminal region consists of a catalytic domain similar to other lysyl oxidases and a cytokine receptor-like domain (1). The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone cofactor (2). LOXL3 is expressed primarily in epidermis, kidney, aorta, and chondrocytes (3). The enzyme is expressed at low levels in most other tissues. Like lysyl oxidase, LOXL3 oxidizes the lysine epsilon -amino groups of a number of collagen substrates, and is also active against other primary amines (4). However, LOXL3 does not require proteolytic processing to be activated.
- Csiszar, K. (2001) Prog. Nucl. Acid Res. Mol. Biol. 70:1.
- Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381.
- Molnar, J. et al. (2003) Biochim. Biophys. Acta 1647:220.
- Lee, J. and Y. Kim (2006) J. Biol. Chem. 281:37282.
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