Human M-CSF Antibody (26730)

R&D Systems | Catalog # MAB216

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization, Cell Culture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 26730
View all M-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human M-CSF

Specificity

Detects human M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse M‑CSF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human M-CSF Antibody (26730)

Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody.

Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody.

Recombinant Human M‑CSF (Catalog # 216-MC) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human M‑CSF (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human M‑CSF Monoclonal Antibody (Catalog # MAB216). The ND50 is typically 0.005-0.020 µg/mL.

Applications for Human M-CSF Antibody (26730)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human M-CSF (Catalog # 216-MC)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND50) is typically 0.005-0.020 µg/mL in the presence of 2.5 ng/mL Recombinant Human M‑CSF.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-CSF

M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Human M-CSF Antibody (26730)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human M-CSF Antibody (26730)

For research use only

Citations for Human M-CSF Antibody (26730)

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