Human Melan-A/MART-1 Antibody

Detection of MART-1 in Human Melanoma​ via seqIF™ staining on COMET™

MART-1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human MART-1, Monoclonal Antibody (Catalog #MAB8008) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Human Melan-A/MART-1 by Western Blot.

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Melan-A/MART-1 Monoclonal Antibody (Catalog # MAB8008) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Melan-A/MART-1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Melan-A/MART-1 in Normal Human Skin.

Melan-A/MART-1 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Melan-A/MART-1 Monoclonal Antibody (Catalog # MAB8008) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to melanocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.