Human Melan-A/MART-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Melan-A/MART-1 by Western Blot.
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Product Details
Supplemental Products

Human Melan-A/MART-1 Antibody Summary

Species Reactivity
Detects human Melan-A/MART-1 in ELISA.
Monoclonal Mouse IgG2A Clone # 872719
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Melan-A/MART-1
Accession # Q16655
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Melan-A/MART-1 antibody by Western Blot. View Larger

Detection of Human Melan-A/MART-1 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Melan-A/MART-1 Monoclonal Antibody (Catalog # MAB8008) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Melan-A/MART-1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Melan-A/MART-1 antibody in Normal Human Skin by Immunohistochemistry (IHC-P). View Larger

Melan-A/MART-1 in Normal Human Skin. Melan-A/MART-1 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Melan-A/MART-1 Monoclonal Antibody (Catalog # MAB8008) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to melanocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melan-A/MART-1

Melan-A (MeLANoma-A; also MART-1, LB39-AA and SK29-AA, MLANA) is an 18-24 kDa member of a melanocyte lineage-specific,  structurally-unrelated family of proteins. It is expressed only in melanocytes, retinal pigment epithelium and melanoma cells. Melan-A is involved in melanosome formation, and appears to stabilize both GPCR143/OA-1 and PMEL, thus ensuring the formation of a stage 2 (melanin production within a fibrillar matrix) melanosome. It is typically found in the Golgi, and upon inversion of its topology, becomes embedded in the ER. It may also appear in endosomes and on the cell surface where, in theory, it is recognizable by a special naïve type of CD8+ T cell that is derived from the thymus. Notably, these cells normally appear to be unresponsive, even in the face of exposure to skin melanocytes during wound healing. Human MLANA is a 118 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 26 aa extracellular region (aa 1-26), and a 71 aa cytoplasmic domain (aa 48-118). There is one utilized phosphorylation site at Ser108, and the molecule is known to undergo acylation. Over aa 52-118, human Melan-A shares 62% aa sequence identity with mouse MLANA.

Entrez Gene IDs
2315 (Human); 77836 (Mouse); 293890 (Rat)
Alternate Names
Antigen LB39-AA; Antigen SK29-AA; Mart 1 Melan A; MART-1; MART1MART-1; MelanA; Melan-A; melanoma antigen recognized by T-cells 1; MLANA; Protein Melan-A

Product Datasheets


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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