Human mGluR5 Antibody

Catalog # Availability Size / Price Qty
MAB45141
MAB45141-SP
mGluR5 in Human Brain.
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Product Details
Citations (2)
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Human mGluR5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human mGLUR in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 464823
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human mGluR5
Ser19-Ser509
Accession # P41594
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry mGluR5 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

mGluR5 in Human Brain. mGluR5 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 25 µg/mL Mouse Anti-Human mGluR5 Monoclonal Antibody (Catalog # MAB45141) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR5

Human metabotropic glutamate receptor 5 (mGluR5; also known as mGluR5b) is a 150 kDa, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length and contains an N-terminal extracellular domain (ECD) of 558 aa. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877-908 in the cytoplasmic tail. Over aa 21-509, human mGluR5 shares 98% aa sequence identity with mouse, rat, and dog mGluR5.

Long Name
Metabotropic Glutamate Receptor 5
Entrez Gene IDs
2915 (Human); 108071 (Mouse); 24418 (Rat)
Alternate Names
glutamate receptor, metabotropic 5; GPRC1E; GPRC1EMGLUR5metabotropic glutamate receptor 5; GRM5; mGlu5; mGluR5

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Citations for Human mGluR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The role of metabotropic glutamate receptor 5 on the stromal cell-derived factor-1/CXCR4 system in oral cancer.
    Authors: Kuribayashi, Nobuyuki, Uchida, Daisuke, Kinouchi, Makoto, Takamaru, Natsumi, Tamatani, Tetsuya, Nagai, Hirokazu, Miyamoto, Youji
    PLoS ONE, 2013;8(11):e80773.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Delayed mGluR5 activation limits neuroinflammation and neurodegeneration after traumatic brain injury.
    Authors: Byrnes KR, Loane DJ, Stoica BA
    J Neuroinflammation, 2012;9(0):43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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