Human metabotropic glutamate receptor 5 (mGluR5; also known as mGluR5b) is a 150 kDa, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length and contains an N-terminal extracellular domain (ECD) of 558 aa. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877-908 in the cytoplasmic tail. Over aa 21-509, human mGluR5 shares 98% aa sequence identity with mouse, rat, and dog mGluR5.
Human mGluR5 Antibody
R&D Systems | Catalog # MAB45141
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry-Paraffin, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 464823
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human mGluR5
Ser19-Ser509
Accession # P41594
Ser19-Ser509
Accession # P41594
Specificity
Detects human mGLUR in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human mGluR5 Antibody
mGluR5 in Human Brain.
mGluR5 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 25 µg/mL Mouse Anti-Human mGluR5 Monoclonal Antibody (Catalog # MAB45141) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse mGluR5 by Immunocytochemistry/Immunofluorescence
mGluR5 is expressed in chronically activated microglia at one month post TBI. (A and B) mGluR5 expression was evaluated in the cortex (A) and hippocampus (B) of sham or TBI brains at one month post-injury. Iba-1 (green) and ED1 (magenta) labeled activated microglia. mGluR5 expression (red) was undetectable in resting microglia that displayed a ramified cellular morphology, but was strongly up-regulated in highly reactive microglia that displayed a hypertrophic or bushy cellular morphology and co-expressed ED1 (merged). (C) The membrane bound component of the NADPH oxidase enzyme, gp91phox (red), co-localized (merged) with ED1-positive reactive microglial (green) at one month post-TBI. Bar = 25 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22373400), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human mGluR5 by Immunocytochemistry/Immunofluorescence
mGluR5 is expressed in chronically activated microglia at one month post TBI. (A and B) mGluR5 expression was evaluated in the cortex (A) and hippocampus (B) of sham or TBI brains at one month post-injury. Iba-1 (green) and ED1 (magenta) labeled activated microglia. mGluR5 expression (red) was undetectable in resting microglia that displayed a ramified cellular morphology, but was strongly up-regulated in highly reactive microglia that displayed a hypertrophic or bushy cellular morphology and co-expressed ED1 (merged). (C) The membrane bound component of the NADPH oxidase enzyme, gp91phox (red), co-localized (merged) with ED1-positive reactive microglial (green) at one month post-TBI. Bar = 25 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22373400), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human mGluR5 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: mGluR5
Long Name
Metabotropic Glutamate Receptor 5
Alternate Names
GPRC1E, GRM5, mGlu5
Gene Symbol
GRM5
UniProt
Additional mGluR5 Products
Product Documents for Human mGluR5 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human mGluR5 Antibody
For research use only
Related Research Areas
Citations for Human mGluR5 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Synaptic Neurotransmission Pathways: Glutamatergic Excitation
