Human mGluR8 Antibody

Catalog # Availability Size / Price Qty
MAB5277
MAB5277-SP
mGluR8 in Human Brain.
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Human mGluR8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human mGluR8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human mGluR1, 2, 3, 4, 5, or 7 is observed.
Source
Monoclonal Mouse IgG2B Clone # 476410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human mGluR8
Gln34-Ser514 (predicted)
Accession # O00222
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Human mGluR8
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry mGluR8 in Human Brain. View Larger

mGluR8 in Human Brain. mGluR8 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human mGluR8 Monoclonal Antibody (Catalog # MAB5277) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR8

mGluR8 is a Group III metabotropic glutamate receptor. These 7-transmembrane glycoproteins are negatively coupled to adenylate cyclase and expressed presynaptically throughout the brain. mGluR8 shows pronounced expression in the dentate gyrus and CA3 regions of the hippocampus, and is thought to play a role in memory formation. Two splice forms, A and B, differ only in the C-terminal 16 amino acids (aa 893‑909), while splice form C diverges at aa 454 and is truncated prior to the first transmembrane segment. Within the N-terminal extracellular region used as an immunogen (aa 34‑514), human, mouse and rat mGluR8 share 98%‑99% aa identity.

Long Name
Metabotropic Glutamate Receptor 8
Entrez Gene IDs
2918 (Human); 14823 (Mouse); 60590 (Rat)
Alternate Names
FLJ41058; glutamate metabotropic receptor 8; glutamate receptor, metabotropic 8; GPRC1H; GPRC1HMGLUR8GLUR8; GRM8; metabotropic glutamate receptor 8; MGC126724; mGlu8; mGluR8

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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