Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 476410
View all mGluR8 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human mGluR8
Gln34-Ser514 (predicted)
Accession # O00222

Specificity

Detects human mGluR8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human mGluR1, 2, 3, 4, 5, or 7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human mGluR8 Antibody

mGluR8 antibody in Human Brain by Immunohistochemistry (IHC-P).

mGluR8 in Human Brain.

mGluR8 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human mGluR8 Monoclonal Antibody (Catalog # MAB5277) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human mGluR8 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Western Blot

1 µg/mL
Sample: Human mGluR8

Reviewed Applications

Read 1 review rated 5 using MAB5277 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR8

mGluR8 is a Group III metabotropic glutamate receptor. These 7-transmembrane glycoproteins are negatively coupled to adenylate cyclase and expressed presynaptically throughout the brain. mGluR8 shows pronounced expression in the dentate gyrus and CA3 regions of the hippocampus, and is thought to play a role in memory formation. Two splice forms, A and B, differ only in the C-terminal 16 amino acids (aa 893‑909), while splice form C diverges at aa 454 and is truncated prior to the first transmembrane segment. Within the N-terminal extracellular region used as an immunogen (aa 34‑514), human, mouse and rat mGluR8 share 98%‑99% aa identity.

Long Name

Metabotropic Glutamate Receptor 8

Alternate Names

GPRC1H, GRM8

Entrez Gene IDs

2918 (Human); 14823 (Mouse); 60590 (Rat)

Gene Symbol

GRM8

UniProt

O00222

Additional mGluR8 Products

Product Documents for Human mGluR8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human mGluR8 Antibody

For research use only

Customer Reviews for Human mGluR8 Antibody (1)

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  • Human mGluR8 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 05/16/2022
    Human mGluR8 Antibody MAB5277

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Protocols

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FAQs

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