Human MIS RII Antibody Summary
Accession # Q16671
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MIS RII Monoclonal Antibody (Catalog # MAB4749).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human MIS RII DuoSet ELISA Kit (Catalog # DY4749-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
MIS RII in Human Ovary. MIS RII was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human MIS RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4749) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human MIS RII ELISA Standard Curve. Recombinant Human MIS RII protein was serially diluted 2-fold and captured by Mouse Anti-Human MIS RII Monoclonal Antibody (Catalog # MAB4749) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MIS RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4749) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIS RII
Human MIS RII (Mullerian inhibiting substance type II receptor), also known as AMHRII (anti-Mullerian hormone type II receptor), is an 82 kDa serine/threonine receptor with a single transmembrane domain that belongs to the family of type II receptors of the TGF-beta superfamily (1). The MIS RII precursor is 573 amino acids in length, with a 17 amino acid (aa) signal sequence, a 127 aa extracellular region that also contains two potential N-linked glycosylation sites, a 26 aa transmembrane region, and a 403 aa cytoplasmic region that contains the serine/threonine kinase domain (1). Human MIS RII shares 82%, 78%, and 77% aa sequence identity with rabbit, mouse, and rat MIS RII, respectively. It is expressed in the mesenchyme surrounding the fetal Mullerian duct, in fetal and postnatal granulosa cells, and in Sertoli cells (1-6). MIS RII is a receptor for Mullerian inhibitor substance (MIS), also known as anti-Mullerian hormone (AMH), which is responsible for regression of the Mullerian duct, the anlagen of the uterus, Fallopian tubes, and upper vagina in male fetuses (1-6). Mutations in MIS RII result in persistent Mullerian duct syndrome (PMDS), an extremely rare form of pseudohermaphroditism (5, 6).
- Imbeaud, S. et al. (1995) Nat. Genet. 11:382.
- Baarends, W.M. et al. (1994) Development 120:189.
- di Clemente, N. et al. (1994) Mol. Endocrinol. 8:1006.
- Teixeira, J. et al. (1996) Endocrinology 137:160.
- Salhi, I et al. (2004) Biochem. J. 379:785.
- Imbeaud, S. et al. (1996) Hum. Mol. Genet. 5:1269.
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