Human MMP-1 Antibody

Catalog # Availability Size / Price Qty
MAB901
MAB901-SP
Human MMP-1 Antibody in Western Blot
4 Images
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (2)

Human MMP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects pro and active forms of human MMP-1. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑2, rhMMP‑3, or rhMMP‑9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 36665
Purification
Protein A or G purified from ascites
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-1
Phe20-Asn469
Accession # P03956
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑1 (Catalog # 901‑MP), see our available Western blot detection antibodies
Knockout Validated
MMP-1 is specifically detected in PC‑3 human prostate cancer parental cell line but is not detectable inMMP-1 knockout PC‑3 cell line.
 
Neutralization
Measured by its ability to neutralize Recombinant Human MMP‑1 (10 µg/mL, Catalog # 901-MP) cleavage of Cultrex Rat Collagen I (250 µg/mL, Catalog # 3440-100-01). The Neutralization Dose (ND50) is typically 200 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human MMP‑1 by Western Blot. View Larger

Detection of Human MMP‑1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MMP‑1 in Human Ovarian Cancer Tissue. View Larger

MMP‑1 in Human Ovarian Cancer Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry MMP‑1 in Human Ovarian Array. View Larger

MMP‑1 in Human Ovarian Array. MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated Western Blot Shows Human MMP‑1 Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human MMP‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of PC-3 human prostate cancer parental cell line and MMP-1 knockout PC-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-1

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,
alpha ‑1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References
  1. Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
Long Name
Matrix Metalloproteinase 1
Entrez Gene IDs
4312 (Human); 83995 (Mouse)
Alternate Names
CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1

Product Datasheets

Citations for Human MMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. The aryl hydrocarbon receptor pathway controls matrix metalloproteinase-1 and collagen levels in human orbital fibroblasts
    Authors: E Roztocil, CL Hammond, MO Gonzalez, SE Feldon, CF Woeller
    Sci Rep, 2020;10(1):8477.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Protective effects of Camellia japonica flower extract against urban air pollutants
    Authors: M Kim, D Son, S Shin, D Park, S Byun, E Jung
    BMC Complement Altern Med, 2019;19(1):30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. The expression of MMP-1 and MMP-9 is up-regulated by smooth muscle cells after their cross-talk with macrophages in high glucose conditions
    Authors: RD Macarie, M Vadana, L Ciortan, MM Tucureanu, A Ciobanu, D Vinereanu, I Manduteanu, M Simionescu, E Butoi
    J. Cell. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression
    Authors: AR Han, TG Lim, YR Song, M Jang, YK Rhee, HD Hong, MH Kim, HJ Kim, CW Cho
    Int J Mol Sci, 2018;19(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. The Effects of the WNT-Signaling Modulators BIO and PKF118-310 on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells
    Authors: X Huang, L Zhong, J Hendriks, JN Post, M Karperien
    Int J Mol Sci, 2018;19(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Co-treatment of TGF-?3 and BMP7 is superior in stimulating chondrocyte redifferentiation in both hypoxia and normoxia compared to single treatments
    Authors: X Huang, L Zhong, JN Post, M Karperien
    Sci Rep, 2018;8(1):10251.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells
    Authors: C Rattanasin, BJ Llewellyn, SE Conrad, KA Gallo
    Oncogenesis, 2017;6(6):e345.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis
    Authors: S Brilha, CWM Ong, B Weksler, N Romero, PO Couraud, JS Friedland
    Sci Rep, 2017;7(1):16031.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer.
    Authors: Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J
    Clin Cancer Res, 2014;20(24):6324-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
    Authors: Li R, Ackerman WE, Mihai C
    PLoS ONE, 2012;7(6):e39766.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010;93(1):86-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009;37(7):849-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2009;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  14. Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
    Authors: Ooi YH, Oh DJ, Rhee DJ
    Invest. Ophthalmol. Vis. Sci., 2009;50(11):5259-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. The association of Sam68 with Vav1 contributes to tumorigenesis.
    Authors: Lazer G, Pe'er L, Schapira V, Richard S, Katzav S
    Cell. Signal., 2007;19(12):2479-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
    Authors: Naveau A, Reinald N, Fournier B
    Connect. Tissue Res., 2007;48(6):300-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Dot Blot
  17. Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis.
    Authors: Lauder SN, Carty SM, Carpenter CE
    Rheumatology (Oxford), 2007;46(5):752-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  18. Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
    Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
    Gut, 2006;55(12):1774-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases.
    Authors: Edwards ST, Cruz AC, Donnelly S, Dazin PF, Schulman ES, Jones KD, Wolters PJ, Hoopes C, Dolganov GM, Fang KC
    J. Pathol., 2005;206(3):279-90.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Tumor necrosis factor-alpha-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.
    Authors: Agren M, Schnabel R, Christensen L, Mirastschijski U
    Eur J Cell Biol, 0;94(1):12-21.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Human MMP-1 Antibody
By Anonymous on 07/13/2018
Application: WB Sample Tested: HeLa cells Species: Human

Human MMP-1 Antibody
By Anonymous on 05/10/2017
Application: WB Sample Tested: PC-3 human prostate cancer cell line Species: Human