Human MMP-1 Antibody
Human MMP-1 Antibody Summary
Phe20-Asn469
Accession # P03956
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MMP‑1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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MMP‑1 in Human Ovarian Cancer Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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MMP‑1 in Human Ovarian Array. MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot Shows Human MMP‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of PC-3 human prostate cancer parental cell line and MMP-1 knockout PC-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-1
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,
alpha ‑1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
- Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
Product Datasheets
Citations for Human MMP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
22
Citations: Showing 1 - 10
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Effect of SPARC Suppression in Mice, Perfused Human Anterior Segments, and Trabecular Meshwork Cells
Authors: WW MacDonald, SS Swaminatha, JY Heo, A Castillejo, J Hsueh, BJ Liu, D Jo, A Du, H Lee, MH Kang, DJ Rhee
Investigative Ophthalmology & Visual Science, 2022;63(6):8.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes
Authors: A Bhattachar, MN Janal, R Veeramacha, I Dolgalev, Z Dubeykovsk, NH Tu, H Kim, S Zhang, AK Wu, M Hagiwara, AR Kerr, MD DeLacure, BL Schmidt, DG Albertson
Sci Rep, 2020;10(1):14724.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The aryl hydrocarbon receptor pathway controls matrix metalloproteinase-1 and collagen levels in human orbital fibroblasts
Authors: E Roztocil, CL Hammond, MO Gonzalez, SE Feldon, CF Woeller
Sci Rep, 2020;10(1):8477.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protective effects of Camellia japonica flower extract against urban air pollutants
Authors: M Kim, D Son, S Shin, D Park, S Byun, E Jung
BMC Complement Altern Med, 2019;19(1):30.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Co-treatment of TGF-?3 and BMP7 is superior in stimulating chondrocyte redifferentiation in both hypoxia and normoxia compared to single treatments
Authors: X Huang, L Zhong, JN Post, M Karperien
Sci Rep, 2018;8(1):10251.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression
Authors: AR Han, TG Lim, YR Song, M Jang, YK Rhee, HD Hong, MH Kim, HJ Kim, CW Cho
Int J Mol Sci, 2018;19(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
The expression of MMP-1 and MMP-9 is up-regulated by smooth muscle cells after their cross-talk with macrophages in high glucose conditions
Authors: RD Macarie, M Vadana, L Ciortan, MM Tucureanu, A Ciobanu, D Vinereanu, I Manduteanu, M Simionescu, E Butoi
J. Cell. Mol. Med., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Effects of the WNT-Signaling Modulators BIO and PKF118-310 on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells
Authors: X Huang, L Zhong, J Hendriks, JN Post, M Karperien
Int J Mol Sci, 2018;19(2):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis
Authors: S Brilha, CWM Ong, B Weksler, N Romero, PO Couraud, JS Friedland
Sci Rep, 2017;7(1):16031.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells
Authors: C Rattanasin, BJ Llewellyn, SE Conrad, KA Gallo
Oncogenesis, 2017;6(6):e345.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer.
Authors: Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J
Clin Cancer Res, 2014;20(24):6324-35.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
Authors: Li R, Ackerman WE, Mihai C
PLoS ONE, 2012;7(6):e39766.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Authors: Cavallo C, Desando G, Facchini A, Grigolo B
J Biomed Mater Res A, 2010;93(1):86-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Exp. Hematol., 2009;37(7):849-56.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Matrix metalloproteinase activity in pediatric acute lung injury.
Authors: Kong MY, Gaggar A, Li Y
Int J Med Sci, 2009;6(1):9-17.
Species: Human
Sample Types: Tissue Secretion
Applications: Western Blot -
Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
Authors: Ooi YH, Oh DJ, Rhee DJ
Invest. Ophthalmol. Vis. Sci., 2009;50(11):5259-65.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The association of Sam68 with Vav1 contributes to tumorigenesis.
Authors: Lazer G, Pe'er L, Schapira V, Richard S, Katzav S
Cell. Signal., 2007;19(12):2479-86.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
Authors: Naveau A, Reinald N, Fournier B
Connect. Tissue Res., 2007;48(6):300-8.
Species: Human
Sample Types: Whole Cells
Applications: Dot Blot -
Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis.
Authors: Lauder SN, Carty SM, Carpenter CE
Rheumatology (Oxford), 2007;46(5):752-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut, 2006;55(12):1774-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases.
Authors: Edwards ST, Cruz AC, Donnelly S, Dazin PF, Schulman ES, Jones KD, Wolters PJ, Hoopes C, Dolganov GM, Fang KC
J. Pathol., 2005;206(3):279-90.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Tumor necrosis factor-alpha-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.
Authors: Agren M, Schnabel R, Christensen L, Mirastschijski U
Eur J Cell Biol, 0;94(1):12-21.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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