hh Human MMP-1 Antibody MAB901: R&D Systems

Human MMP-1 Antibody

(12 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects pro and active forms of human MMP-1. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑2, rhMMP‑3, or rhMMP‑9 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 36665
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human MMP-1
    Phe20-Asn469
    Accession # P03956
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human MMP‑1 (Catalog # 901‑MP), see our available Western blot detection antibodies
  • Knockout Validated
    MMP-1 is specifically detected in PC‑3 human prostate cancer parental cell line but is not detectable inMMP-1 knockout PC‑3 cell line.
  • Neutralization
    Measured by its ability to neutralize Recombinant Human MMP‑1 (10 µg/mL, Catalog # 901-MP) cleavage of Cultrex Rat Collagen I (250 µg/mL, Catalog # 3440-100-01). The Neutralization Dose (ND50) is typically 200 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MMP‑1 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP‑1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
MMP‑1 in Human Ovarian Cancer Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
MMP‑1 in Human Ovarian Array. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Knockout Validated
Western Blot Shows Human MMP‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of PC‑3 human prostate cancer parental cell line and MMP-1 knockout PC‑3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑1 at approximately 50 kDa (as indicated) in the parental PC‑3cell line, but is not detectable in knockout PC‑3 cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-1

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,
alpha ‑1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

  • References:
    1. Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
  • Long Name:
    Matrix Metalloproteinase 1
  • Entrez Gene IDs:
    4312 (Human)
  • Alternate Names:
    CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells
    Authors: C Rattanasin, BJ Llewellyn, SE Conrad, KA Gallo
    Oncogenesis, 2017;6(6):e345.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer.
    Authors: Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J
    Clin Cancer Res, 2014;20(24):6324-35.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
    Authors: Li R, Ackerman WE, Mihai C
    PLoS ONE, 2012;7(6):e39766.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010;93(1):86-95.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
    Authors: Ooi YH, Oh DJ, Rhee DJ
    Invest. Ophthalmol. Vis. Sci., 2009;50(11):5259-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2009;6(1):9-17.
    Species: Human
    Sample Type: Tissue Secretion
    Application: WB
  7. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009;37(7):849-56.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
    Authors: Naveau A, Reinald N, Fournier B
    Connect. Tissue Res., 2007;48(6):300-8.
    Species: Human
    Sample Type: Whole Cells
    Application: DB
  9. Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis.
    Authors: Lauder SN, Carty SM, Carpenter CE
    Rheumatology (Oxford), 2007;46(5):752-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  10. The association of Sam68 with Vav1 contributes to tumorigenesis.
    Authors: Lazer G, Pe'er L, Schapira V, Richard S, Katzav S
    Cell. Signal., 2007;19(12):2479-86.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
    Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
    Gut, 2006;55(12):1774-80.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases.
    Authors: Edwards ST, Cruz AC, Donnelly S, Dazin PF, Schulman ES, Jones KD, Wolters PJ, Hoopes C, Dolganov GM, Fang KC
    J. Pathol., 2005;206(3):279-90.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
Expand to show all 12 Citations
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Primary Antibodies
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Human MMP-1 Antibody

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse IgG Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationWestern Blot
Sample TestedPC-3 human prostate cancer cell line
SpeciesHuman

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