Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1
Ser2-Ser501
Accession # P00352

Specificity

Detects human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in direct ELISAs and detects human and mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody

Detection of Human and Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody by Western Blot.

Detection of Human and Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 by Western Blot.

Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 by Simple WesternTM.

Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (Human Urine) (NBP2-49840) and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 56 kDa (as indicated) using 10 µg/ml of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Aldehyde Dehydrogenase 1-A1/ALDH1A1 in HeLa Human Cell Line.

Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in Human Prostate Cancer Tissue.

Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody by Simple WesternTM.

Detection of Human and Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 57 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Aldehyde Dehydrogenase 1‑A1 (Catalog # 5869-DH), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685), Exosome Standards (Human Urine) (Catalog # NBP2-49840), HepG2 human hepatocellular carcinoma cell line, Human liver tissue and mouse liver tissue

Western Blot

1 µg/mL
Sample: Human liver tissue, human testis tissue, and mouse liver tissue

Reviewed Applications

Read 1 review rated 5 using AF5869 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).

References

  1. Marchitti, S.A. et al. (2008) Expert Opin. Drug Metab. Toxicol. 4:697.
  2. Zhao, D. et al. (1996) Eur. J. Biochem. 240:15.
  3. King, G. and R. Holmes (1997) Adv. Exp. Med. Biol. 414:19.
  4. Jacobs, F.M. et al. (2007) Development 134:2673.
  5. Chute, J.P. et al. (2006) Proc. Natl. Acad. Sci. USA 103:11707.
  6. Ueshima, Y. et al. (1993) Alcohol Alcohol. 1B:15.

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Entrez Gene IDs

216 (Human)

Gene Symbol

ALDH1A1

UniProt

P00352

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody

For research use only

Citations for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody

Customer Reviews for Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody
    Name: Dharmaraja Allimuthu
    Application: Western Blot
    Sample Tested: RPMI 8226 human multiple myeloma cell line
    Species: Human
    Verified Customer | Posted 01/10/2018
    Human/Mouse Aldehyde Dehydrogenase 1‑A1/ ALDH1A1 Antibody AF5869

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies