Human/Mouse ATG7 Antibody
Human/Mouse ATG7 Antibody Summary
Asn387-Gln570
Accession # O95352
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ATG7 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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ATG7 in RAW 264.7 Mouse Cell Line. ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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ATG7 in Human Brain. ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Human ATG7 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATG7
ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.
Product Datasheets
Citations for Human/Mouse ATG7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Autolysosomes and caspase-3 control the biogenesis and release of immunogenic apoptotic exosomes
Authors: D Beillevair, F Migneault, J Turgeon, D Gingras, AK Rimbaud, G Marcoux, C Spino, N Thibodeau, E Bonneil, P Thibault, É Boilard, M Dieudé, MJ Hébert
Cell Death & Disease, 2022;13(2):145.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Autophagy drives fibroblast senescence through MTORC2 regulation
Authors: M Bernard, B Yang, F Migneault, J Turgeon, M Dieudé, MA Olivier, GB Cardin, M El-Diwany, K Underwood, F Rodier, MJ Hébert
Autophagy, 2020;0(0):1-13.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Autophagy coupled to translation is required for long-term memory
Authors: K Pandey, XW Yu, A Steinmetz, CM Alberini
Autophagy, 2020;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
A Nucleotide Analog Prevents Colitis-Associated Cancer via Beta-Catenin Independently of Inflammation and Autophagy
Authors: YH Sheng, R Giri, J Davies, V Schreiber, S Alabbas, R Movva, Y He, A Wu, J Hooper, B McWhinney, I Oancea, G Kijanka, S Hasnain, AJ Lucke, DP Fairlie, MA McGuckin, TH Florin, J Begun
Cell Mol Gastroenterol Hepatol, 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits
Authors: S Jung, S Choe, H Woo, H Jeong, HK An, H Moon, HY Ryu, BK Yeo, YW Lee, H Choi, JY Mun, W Sun, HK Choe, EK Kim, SW Yu
Autophagy, 2019;0(0):1-19.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Histone deacetylase 3 is required for iNKT cell development
Authors: P Thapa, S Romero Aro, JY Chung, DB Sant'Angel, VS Shapiro
Sci Rep, 2017;7(1):5784.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia
Authors: Maurizio Bossola
Exp. Gerontol., 2016;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF.
Authors: Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M
Autophagy, 2014;10(12):2193-207.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Average Rating: 5 (Based on 2 Reviews)
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