Human/Mouse ATG7 Antibody

Catalog # Availability Size / Price Qty
MAB6608
MAB6608-SP
Detection of Human ATG7 by Western Blot.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse ATG7 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human ATG7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 683906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ATG7
Asn387-Gln570
Accession # O95352
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human ATG7 antibody by Western Blot. View Larger

Detection of Human ATG7 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ATG7 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

ATG7 in Human Brain. ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry ATG7 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

ATG7 in RAW 264.7 Mouse Cell Line. ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry Detection of ATG7 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Human ATG7 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human ATG7 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG7

ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.

Long Name
ATG7 Autophagy Related 7 Homolog
Entrez Gene IDs
10533 (Human); 74244 (Mouse); 312647 (Rat); 856576 (Yeast)
Alternate Names
APG7 autophagy 7-like (S. cerevisiae); APG7; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7

Product Datasheets

Citations for Human/Mouse ATG7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Autophagy drives fibroblast senescence through MTORC2 regulation
    Authors: M Bernard, B Yang, F Migneault, J Turgeon, M Dieudé, MA Olivier, GB Cardin, M El-Diwany, K Underwood, F Rodier, MJ Hébert
    Autophagy, 2020;0(0):1-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Autophagy coupled to translation is required for long-term memory
    Authors: K Pandey, XW Yu, A Steinmetz, CM Alberini
    Autophagy, 2020;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. A Nucleotide Analog Prevents Colitis-Associated Cancer via Beta-Catenin Independently of Inflammation and Autophagy
    Authors: YH Sheng, R Giri, J Davies, V Schreiber, S Alabbas, R Movva, Y He, A Wu, J Hooper, B McWhinney, I Oancea, G Kijanka, S Hasnain, AJ Lucke, DP Fairlie, MA McGuckin, TH Florin, J Begun
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits
    Authors: S Jung, S Choe, H Woo, H Jeong, HK An, H Moon, HY Ryu, BK Yeo, YW Lee, H Choi, JY Mun, W Sun, HK Choe, EK Kim, SW Yu
    Autophagy, 2019;0(0):1-19.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Histone deacetylase 3 is required for iNKT cell development
    Authors: P Thapa, S Romero Aro, JY Chung, DB Sant'Angel, VS Shapiro
    Sci Rep, 2017;7(1):5784.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia
    Authors: Maurizio Bossola
    Exp. Gerontol., 2016;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF.
    Authors: Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M
    Autophagy, 2014;10(12):2193-207.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse ATG7 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse ATG7 Antibody
By Anonymous on 03/23/2022
Application: Immunohistochemistry Sample Tested: Brain tissue Species: Mouse

Human/Mouse ATG7 Antibody
By Anonymous on 09/07/2021
Application: Immunohistochemistry Sample Tested: Ocular melanoma Species: Human