Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 320831
View all c-Jun Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human c-Jun
Met1-Phe331
Accession # P05412

Specificity

Detects human and mouse c-Jun in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse c‑Jun Antibody

Detection of Human c‑Jun antibody by Western Blot.

Detection of Human c‑Jun by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for the indicated time. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse c-Jun Monoclonal Antibody (Catalog # MAB2670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for c-Jun was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

c-Jun antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

c‑Jun in HeLa Human Cell Line.

c-Jun was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse c-Jun Monoclonal Antibody (Catalog # MAB2670) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse c‑Jun Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Reviewed Applications

Read 1 review rated 5 using MAB2670 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: c-Jun

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Entrez Gene IDs

3725 (Human)

Gene Symbol

JUN

UniProt

P05412

Additional c-Jun Products

Product Documents for Human/Mouse c‑Jun Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse c‑Jun Antibody

For research use only

Citations for Human/Mouse c‑Jun Antibody

Customer Reviews for Human/Mouse c‑Jun Antibody (1)

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  • Human/Mouse c-Jun Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa human cervical epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 02/24/2022
    Human/Mouse c‑Jun Antibody MAB2670

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Protocols

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