Human/Mouse c-Jun Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse c-Jun in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 320831
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human c-Jun
    Met1-Phe331
    Accession # P05412
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human c‑Jun by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for the indicated time. Gels were loaded with 20 μg of cytoplasmic (Cyto) and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse c‑Jun Monoclonal Antibody (Catalog # MAB2670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for c‑Jun was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
c‑Jun in HeLa Human Cell Line. c‑Jun was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse c‑Jun Monoclonal Antibody (Catalog # MAB2670) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Jun
c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.
  • Long Name:
    Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1
  • Entrez Gene IDs:
    3725 (Human)
  • Alternate Names:
    Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog
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