Human/Mouse c-Jun Antibody

Catalog # Availability Size / Price Qty
Detection of Human c‑Jun by Western Blot.
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Product Details
Citations (1)
Supplemental Products
Reviews (1)

Human/Mouse c-Jun Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse c-Jun in Western blots.
Monoclonal Mouse IgG1 Clone # 320831
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human c-Jun
Accession # P05412
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human c‑Jun antibody by Western Blot. View Larger

Detection of Human c‑Jun by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for the indicated time. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse c-Jun Monoclonal Antibody (Catalog # MAB2670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for c-Jun was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry c-Jun antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

c‑Jun in HeLa Human Cell Line. c-Jun was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse c-Jun Monoclonal Antibody (Catalog # MAB2670) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Jun

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Long Name
Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1
Entrez Gene IDs
3725 (Human)
Alternate Names
Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog

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Citation for Human/Mouse c-Jun Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Indirubin 3'-Oxime Inhibits Migration, Invasion, and Metastasis InVivo in Mice Bearing Spontaneously Occurring Pancreatic Cancer via Blocking the RAF/ERK, AKT, and SAPK/JNK Pathways
    Authors: Y Ichimaru, M Sano, I Kajiwara, T Tobe, H Yoshioka, K Hayashi, H Ijichi, S Miyairi
    Transl Oncol, 2019;12(12):1574-1582.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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Human/Mouse c-Jun Antibody
By Anonymous on 02/24/2022
Application: WB Sample Tested: HeLa human cervical epithelial carcinoma cell line Species: Human