Human/Mouse Carm1 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human Carm1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Carm1
    Lys209-Leu379
    Accession # Q86X55
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Carm1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carm1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Carm1 in HeLa Human Cell Line. Carm1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7277) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carm1

Carm1 (Coactivator-associated arginine methyltransferase 1; also PRMT4) is a 60-64 kDa member of the Arg N-methyltransferase family of enzymes. It is ubiquitously expressed, and found in the cytoplasm during mitosis, and in the nucleus during the G1, G2 and S phases of the cell cycle. Carm1 binds to nuclear receptor p160 family coactivators. When bound, it methylates DNA-associated histone H3 arginines, allowing for chromatin remodeling and gene activation. It also plays a role in pre-mRNA splicing through its methylation of splicing factors, and regulates the stability of RNA-binding proteins. Human Carm1 is 608 amino acids (aa) in length. It contains one catalytic site between aa 184-394, and a transactivation domain at the C-terminus (aa 499-608). There is one automethylation site at Arg550, and a phosphorylation site at Ser216 that, when utilized, promotes cytosolic localization. Carm1 likely forms homodimers. There are three potential isoform variants. One shows an alternative start site at Met378, a second possesses a 16 aa substitution for aa 369-608, and a third contains a deletion of aa 539-561. Over aa 209-379, human and mouse Carm1 are identical in aa sequence.

  • Long Name:
    Coactivator-associated Arginine Methyltransferase 1
  • Entrez Gene IDs:
    10498 (Human); 59035 (Mouse); 363026 (Rat)
  • Alternate Names:
    Carm1; coactivator-associated arginine methyltransferase 1EC 2.1.1.-; EC 2.1.1; histone-arginine methyltransferase CARM1; PRMT4; PRMT4EC 2.1.1.125; Protein arginine N-methyltransferase 4
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