Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EZH2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EZH2
Gly512-Ile645
Accession # Q15910

Specificity

Detects human and mouse EZH2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse EZH2 Antibody

Detection of Human and Mouse EZH2 antibody by Western Blot.

Detection of Human and Mouse EZH2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse EZH2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EZH2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human EZH2/KMT6 by Western Blot

Detection of Human EZH2/KMT6 by Western Blot

Measurement of EZH2 protein under conditions of SIRT1 knockdown. SIRT1 knockdown in Caco-2 cells was achieved using two different siRNAs and protein was analysed by Western blotting using anti-EZH2 antibody (a) or anti-SIRT1 antibody (to confirm efficacy of knockdown) (b). Blots were probed with anti-alpha -tubulin antibody to confirm equal protein loading and transfer. Approximately 10 μg of protein was loaded in each lane. Three biological replicates are presented for each condition. Approximate molecular weights are indicated Image collected and cropped by CiteAb from the following publication (https://humgenomics.biomedcentral.com/articles/10.1186/s40246-015-0036-0), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human EZH2/KMT6 by Western Blot

Detection of Human EZH2/KMT6 by Western Blot

EZH2 elevates at S phase in hDPCs and interacts with PCNA. a Schematic chart illustrating the serum deprivation approach used for G0/G1 phase synchronization of hDPCs. b Western blot shows the expression of EZH2 in hDPCs at the indicated time points after release from serum deprivation. Immunofluorescence shows the subcellular localization of EZH2 in hDPCs in G1 or S phase. c Co-immunoprecipitation (Co-IP) of EZH2 and PCNA in hDPCs (synchronized at S phase) and proximity ligation assay (PLA) after co-incubating anti-EZH2 and anti-PCNA antibodies in hDPCs in S phase. IgGs were used as control antibodies for the IP. Antibodies used for IP and western blot are labelled as IP and IB, respectively. Total lysate (10 μg) was used as an input control. Scale bars represent 20 μm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30071900), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human EZH2/KMT6 by Proximity Ligation Assay

Detection of Human EZH2/KMT6 by Proximity Ligation Assay

EZH2 elevates at S phase in hDPCs and interacts with PCNA. a Schematic chart illustrating the serum deprivation approach used for G0/G1 phase synchronization of hDPCs. b Western blot shows the expression of EZH2 in hDPCs at the indicated time points after release from serum deprivation. Immunofluorescence shows the subcellular localization of EZH2 in hDPCs in G1 or S phase. c Co-immunoprecipitation (Co-IP) of EZH2 and PCNA in hDPCs (synchronized at S phase) and proximity ligation assay (PLA) after co-incubating anti-EZH2 and anti-PCNA antibodies in hDPCs in S phase. IgGs were used as control antibodies for the IP. Antibodies used for IP and western blot are labelled as IP and IB, respectively. Total lysate (10 μg) was used as an input control. Scale bars represent 20 μm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30071900), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human EZH2/KMT6 by Western Blot

Detection of Human EZH2/KMT6 by Western Blot

Measurement of EZH2 protein under conditions of SIRT1 knockdown. SIRT1 knockdown in Caco-2 cells was achieved using two different siRNAs and protein was analysed by Western blotting using anti-EZH2 antibody (a) or anti-SIRT1 antibody (to confirm efficacy of knockdown) (b). Blots were probed with anti-alpha -tubulin antibody to confirm equal protein loading and transfer. Approximately 10 μg of protein was loaded in each lane. Three biological replicates are presented for each condition. Approximate molecular weights are indicated Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26104761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse EZH2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HL-60 human acute promyelocytic leukemia cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and mouse spleen tissue

Reviewed Applications

Read 1 review rated 5 using AF4767 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EZH2

EZH2 (Enhancer of zeste homolog 2; also ENX-1 and Lys N-methyltransferase 6) is an 80 kDa member of the EZ family of chromatin-dependent gene regulators. It is a nuclear protein that represses gene transcription through histone methylation. Human EZH2 is 746 amino acids (aa) in length. It contains an NLS (aa 490‑495), a Cys-rich region, and a methyltransferase SET (Suppressor/Enhancer/Trithorax) domain (aa 606‑729). There are four potential splice variants. One shows a premature truncation after Cys286, a second shows a 6 aa substitution for aa 329‑746, a third shows a deletion of aa 83‑121, and the fourth exhibits a 5 aa insertion after His297. Over aa 512‑645, human, mouse and canine EZH2 are identical in amino acid sequence.

Long Name

Enhancer of Zeste Homolog 2 [Drosophila]

Alternate Names

ENX1, KMT6

Entrez Gene IDs

2146 (Human); 14056 (Mouse); 312299 (Rat)

Gene Symbol

EZH2

UniProt

Q15910

Additional EZH2 Products

Product Documents for Human/Mouse EZH2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse EZH2 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Epigenetics

Citations for Human/Mouse EZH2 Antibody

Customer Reviews for Human/Mouse EZH2 Antibody (1)

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  • Human/Mouse EZH2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 10/26/2021
    Human/Mouse EZH2 Antibody AF4767

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