Detection of Human and Mouse EZH2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse EZH2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EZH2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
EZH2 (Enhancer of zeste homolog 2; also ENX-1 and Lys N-methyltransferase 6) is an 80 kDa member of the EZ family of chromatin-dependent gene regulators. It is a nuclear protein that represses gene transcription through histone methylation. Human EZH2 is 746 amino acids (aa) in length. It contains an NLS (aa 490‑495), a Cys-rich region, and a methyltransferase SET (Suppressor/Enhancer/Trithorax) domain (aa 606‑729). There are four potential splice variants. One shows a premature truncation after Cys286, a second shows a 6 aa substitution for aa 329‑746, a third shows a deletion of aa 83‑121, and the fourth exhibits a 5 aa insertion after His297. Over aa 512‑645, human, mouse and canine EZH2 are identical in amino acid sequence.
Enhancer of Zeste Homolog 2 [Drosophila]
Entrez Gene IDs:
2146 (Human); 14056 (Mouse); 312299 (Rat)
EC 2.1.1; EC 184.108.40.206; enhancer of zeste (Drosophila) homolog 2; enhancer of zeste 2; enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste homolog 2; ENX1; ENX-1KMT6EZH1KMT6A; EZH2; histone-lysine N-methyltransferase EZH2; KMT6; Lysine N-methyltransferase 6; MGC9169
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