Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Fish - Oryzias latipes (Medaka or Japanese Rice Fish)

Applications

Validated:

Knockout Validated, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 647113
View all Fatty Acid Synthase/FASN Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Fatty Acid Synthase/FASN
Ser10-Cys212
Accession # P49327

Specificity

Detects human and mouse Fatty Acid Synthase/FASN in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse Fatty Acid Synthase/FASN Antibody

Detection of Human and Mouse Fatty Acid Synthase/FASN antibody by Western Blot.

Detection of Human and Mouse Fatty Acid Synthase/FASN by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western Blot Shows Human Fatty Acid Synthase/FASN Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Fatty Acid Synthase/FASN Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Fatty Acid Synthase/FASN knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fatty Acid Synthase/FASN at approximately 275 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Fatty Acid Synthase/FASN by Simple WesternTM.

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Fatty Acid Synthase/FASN at approximately 213 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Applications for Human/Mouse Fatty Acid Synthase/FASN Antibody

Application
Recommended Usage

Knockout Validated

Fatty Acid Synthase/FASN is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Fatty Acid Synthase/FASN knockout HEK293T cell line.

Simple Western

20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and mouse liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fatty Acid Synthase/FASN

FASN (Fatty Acid Synthase; also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a  beta -ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta -ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92% aa identity with mouse FASN.

Alternate Names

FASN, OA-519, SDR27X1

Entrez Gene IDs

2194 (Human); 14104 (Mouse)

Gene Symbol

FASN

UniProt

P49327 (Human)

Additional Fatty Acid Synthase/FASN Products

Product Documents for Human/Mouse Fatty Acid Synthase/FASN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Fatty Acid Synthase/FASN Antibody

For research use only

Citations for Human/Mouse Fatty Acid Synthase/FASN Antibody

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