Detection of Human/Mouse GATA‑2 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA‑2 Monoclonal Antibody (Catalog # MAB2046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GATA‑2 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues. Specifically, GATA-2 is expressed in hematopoietic progenitors and stem cells. GATA-2 expression is associated with cell proliferation. GATA-2 is 480 amino acids in length and contains 2 Zn finger domains responsible for DNA binding (aa 295‑319 and 349‑373). Over aa 15‑279 human GATA-2 share 96% identity with mouse GATA-2.
Entrez Gene IDs:
endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA2; GATA-2; GATA-binding protein 2NFE1B; MGC2306
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