Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 527530
View all GATA-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769

Specificity

Detects endogenous human and mouse GATA-2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse GATA‑2 Antibody

Detection of Human/Mouse GATA‑2 antibody by Western Blot.

Detection of Human/Mouse GATA‑2 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA-2 Monoclonal Antibody (Catalog # MAB2046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GATA-2 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse GATA‑2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and DA3 mouse myeloma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-2

GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues. Specifically, GATA-2 is expressed in hematopoietic progenitors and stem cells. GATA-2 expression is associated with cell proliferation. GATA-2 is 480 amino acids in length and contains 2 Zn finger domains responsible for DNA binding (aa 295‑319 and 349‑373). Over aa 15‑279 human GATA-2 share 96% identity with mouse GATA-2.

Alternate Names

GATA2

Entrez Gene IDs

2624 (Human)

Gene Symbol

GATA2

UniProt

P23769

Additional GATA-2 Products

Product Documents for Human/Mouse GATA‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse GATA‑2 Antibody

For research use only

Citations for Human/Mouse GATA‑2 Antibody

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