Human/Mouse GLP/EHMT1 Antibody

R&D Systems | Catalog # PP-B0422-00

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Direct ELISA, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # B0422
View all GLP/EHMT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse GLP/EHMT1
aa 134-234

Specificity

This antibody specifically recognizes mouse GLP and cross-reacts with human GLP. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human/Mouse GLP/EHMT1 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect mouse GLP.

Immunohistochemistry

This antibody can be used at 5 μg/mL with the appropriate secondary reagents to detect mouse GLP.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 0.5 μg/mL with the appropriate secondary reagents to detect mouse GLP.

Reviewed Applications

Read 8 reviews rated 4.3 using PP-B0422-00 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: GLP/EHMT1

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1.

Long Name

G9a-like Protein 1/Euchromatic Histone-Lysine N-Methyltransferase 1

Alternate Names

DEL9q34, EHMT1, Eu-HMTase1, KMT1D, Lysine N-methyltransferase 1D

Entrez Gene IDs

79813 (Human); 77683 (Mouse)

Gene Symbol

EHMT1

Additional GLP/EHMT1 Products

Product Documents for Human/Mouse GLP/EHMT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse GLP/EHMT1 Antibody

For research use only

Related Research Areas

Epigenetics
Notch Pathway

Citations for Human/Mouse GLP/EHMT1 Antibody

Customer Reviews for Human/Mouse GLP/EHMT1 Antibody (8)

4.3 out of 5
8 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 8 reviews Showing All
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  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 09/07/2021
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Chromatin Immunoprecipitation
    Sample Tested: B cells
    Species: Human
    Verified Customer | Posted 06/26/2018
    ChIP was performed using PP-B0422-00 or isotype IgG (1 ug antibody/ 100 ug Chromatin). Realtime-PCR was performed using primers specific for promoter of gene of interest and the results are expressed as % input.
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T
    Species: Human
    Verified Customer | Posted 06/22/2018
    293T whole cell lysate was immunoblotted with anti GLP anitibody.
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: CCD-1070Sk human foreskin fibroblast cell line
    Species: Human
    Verified Customer | Posted 06/19/2018
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RL human non-Hodgkin's lymphoma B cell line
    Species: Human
    Verified Customer | Posted 01/04/2018
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human Dermal Microvascular Endothelial cells - Paraformaldehyde fixed cells
    Species: Human
    Verified Customer | Posted 07/11/2017
    Immunofluorescence staining of paraformaldehyde fixed Human Dermal Microvascular Endothelial cells. PP-B0422-00 used at 1:100 dilution.
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
    Species: Human
    Verified Customer | Posted 04/21/2017
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
    Species: Human
    Verified Customer | Posted 04/21/2017
    Human/Mouse GLP/EHMT1 Antibody PP-B0422-00

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail